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Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.

Publication ,  Journal Article
Jing, H; Korasick, DA; Emenecker, RJ; Morffy, N; Wilkinson, EG; Powers, SK; Strader, LC
Published in: Nature communications
July 2022
Published version (DOI)

Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCFAFF1 revealed that this E3 ubiquitin ligase directly interacts with ARF19 and ARF7 to regulate their accumulation, condensation, and nucleo-cytoplasmic partitioning. Mutants defective in AFF1 display attenuated auxin responsiveness, and developmental defects, suggesting that SCFAFF1 -mediated regulation of ARF protein drives aspects of auxin response and plant development.

Duke Scholars

Lucia Carol Strader
Author Lucia Carol Strader Biology
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Published In

Nature communications

DOI

10.1038/s41467-022-31628-2

EISSN

2041-1723

ISSN

2041-1723

Publication Date

July 2022

Volume

13

Issue

1

Start / End Page

4015

Related Subject Headings

  • Plant Roots
  • Indoleacetic Acids
  • Gene Expression Regulation, Plant
  • Arabidopsis Proteins
  • Arabidopsis
 

Citation

APA
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Jing, H., Korasick, D. A., Emenecker, R. J., Morffy, N., Wilkinson, E. G., Powers, S. K., & Strader, L. C. (2022). Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning. Nature Communications, 13(1), 4015. https://doi.org/10.1038/s41467-022-31628-2
Jing, Hongwei, David A. Korasick, Ryan J. Emenecker, Nicholas Morffy, Edward G. Wilkinson, Samantha K. Powers, and Lucia C. Strader. “Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.” Nature Communications 13, no. 1 (July 2022): 4015. https://doi.org/10.1038/s41467-022-31628-2.
Jing H, Korasick DA, Emenecker RJ, Morffy N, Wilkinson EG, Powers SK, et al. Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning. Nature communications. 2022 Jul;13(1):4015.
Jing, Hongwei, et al. “Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.” Nature Communications, vol. 13, no. 1, July 2022, p. 4015. Epmc, doi:10.1038/s41467-022-31628-2.
Jing H, Korasick DA, Emenecker RJ, Morffy N, Wilkinson EG, Powers SK, Strader LC. Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning. Nature communications. 2022 Jul;13(1):4015.

Published In

Nature communications

DOI

10.1038/s41467-022-31628-2

EISSN

2041-1723

ISSN

2041-1723

Publication Date

July 2022

Volume

13

Issue

1

Start / End Page

4015

Related Subject Headings

  • Plant Roots
  • Indoleacetic Acids
  • Gene Expression Regulation, Plant
  • Arabidopsis Proteins
  • Arabidopsis