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[Expression of recombinant human hemangiopoietin and preparation of its polyclonal antibody].

Publication ,  Journal Article
Ren, Q; Liu, Y-J; Xu, B; Han, Z-B; Lu, S-H; Ma, F-X; Chen, Z; Han, Z-C
Published in: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
November 2006
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AIM: To express the recombinant fusion protein of hemangiopoietin (HAPO) and prepare the rabbit-anti-human HAPO polyclonal antibody. METHODS: The sequence encoding HAPO was amplified by PCR and cloned into plasmid pET32c to construct recombinant prokaryotic expression system. The recombinant expression vectors were identified by enzyme digestion analysis and transformed into E. coli. The HAPO protein was purified by affinity chromatography. Rabbits were immunized with the HAPO protein, and the immune sera of rabbits were collected. Antibodies (IgG) obtained from the immune sera were purified. RESULTS: The purified HAPO protein was successfully obtained. The purified polyclonal antibody of rabbit-anti-human HAPO was also obtained from the immune sera of rabbits, and could response to human HAPO. CONCLUSION: A prokaryotic expression system of human HAPO has been prepared and the polyclonal antibody against HAPO has been prepared, which can be used to determine HAPO protein.

Duke Scholars

Zhong Chen
Author Zhong Chen Pathology

Published In

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi

ISSN

1007-8738

Publication Date

November 2006

Volume

22

Issue

6

Start / End Page

801 / 803

Location

China

Related Subject Headings

  • Recombinant Proteins
  • Proteoglycans
  • Plasmids
  • Immunology
  • Immune Sera
  • Hydrogen-Ion Concentration
  • Humans
  • Escherichia coli
  • Electrophoresis, Polyacrylamide Gel
  • DNA Restriction Enzymes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ren, Q., Liu, Y.-J., Xu, B., Han, Z.-B., Lu, S.-H., Ma, F.-X., … Han, Z.-C. (2006). [Expression of recombinant human hemangiopoietin and preparation of its polyclonal antibody]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 22(6), 801–803.
Ren, Qian, Yong-Jun Liu, Bin Xu, Zhi-Bo Han, Shi-Hong Lu, Feng-Xia Ma, Zhong Chen, and Zhong-Chao Han. “[Expression of recombinant human hemangiopoietin and preparation of its polyclonal antibody].” Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 22, no. 6 (November 2006): 801–3.
Ren Q, Liu Y-J, Xu B, Han Z-B, Lu S-H, Ma F-X, et al. [Expression of recombinant human hemangiopoietin and preparation of its polyclonal antibody]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Nov;22(6):801–3.
Ren, Qian, et al. “[Expression of recombinant human hemangiopoietin and preparation of its polyclonal antibody].” Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, vol. 22, no. 6, Nov. 2006, pp. 801–03.
Ren Q, Liu Y-J, Xu B, Han Z-B, Lu S-H, Ma F-X, Chen Z, Han Z-C. [Expression of recombinant human hemangiopoietin and preparation of its polyclonal antibody]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Nov;22(6):801–803.

Published In

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi

ISSN

1007-8738

Publication Date

November 2006

Volume

22

Issue

6

Start / End Page

801 / 803

Location

China

Related Subject Headings

  • Recombinant Proteins
  • Proteoglycans
  • Plasmids
  • Immunology
  • Immune Sera
  • Hydrogen-Ion Concentration
  • Humans
  • Escherichia coli
  • Electrophoresis, Polyacrylamide Gel
  • DNA Restriction Enzymes