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p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity.

Publication ,  Journal Article
Vahebi, S; Ota, A; Li, M; Warren, CM; de Tombe, PP; Wang, Y; Solaro, RJ
Published in: Circ Res
February 16, 2007

Our objective in work presented here was to understand the mechanisms by which activated p38alpha MAPK depresses myocardial contractility. To test the hypothesis that activation of p38 MAPK directly influences sarcomeric function, we used transgenic mouse models with hearts in which p38 MAPK was constitutively turned on by an upstream activator (MKK6bE). These hearts demonstrated a significant depression in ejection fraction after induction of the transgene. We also studied hearts of mice expressing a dominant negative p38alpha MAPK. Simultaneous determination of tension and ATPase activity of detergent-skinned fiber bundles from left ventricular papillary muscle demonstrated a significant inhibition of both maximum tension and ATPase activity in the transgenic-MKK6bE hearts. Fibers from hearts expressing dominant negative p38alpha MAPK demonstrated no significant change in tension or ATPase activity. There were no significant changes in phosphorylation level of troponin-T3 and troponin-T4, or myosin light chain 2. However, compared with controls, there was a significant depression in levels of phosphorylation of alpha-tropomyosin and troponin I in fiber bundles from transgenic-MKK6bE hearts, but not from dominant negative p38alpha MAPK hearts. Our experiments also showed that p38alpha MAPK colocalizes with alpha-actinin at the Z-disc and complexes with protein phosphatases (PP2alpha, PP2beta). These data are the first to indicate that chronic activation of p38alpha MAPK directly depresses sarcomeric function in association with decreased phosphorylation of alpha-tropomyosin.

Duke Scholars

Published In

Circ Res

DOI

EISSN

1524-4571

Publication Date

February 16, 2007

Volume

100

Issue

3

Start / End Page

408 / 415

Location

United States

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Troponin I
  • Tropomyosin
  • Transgenes
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Sarcomeres
  • Recombinant Fusion Proteins
  • Protein Transport
  • Protein Processing, Post-Translational
 

Citation

APA
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ICMJE
MLA
NLM
Vahebi, S., Ota, A., Li, M., Warren, C. M., de Tombe, P. P., Wang, Y., & Solaro, R. J. (2007). p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity. Circ Res, 100(3), 408–415. https://doi.org/10.1161/01.RES.0000258116.60404.ad
Vahebi, Susan, Asuka Ota, Manxiang Li, Chad M. Warren, Pieter P. de Tombe, Yibin Wang, and R John Solaro. “p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity.Circ Res 100, no. 3 (February 16, 2007): 408–15. https://doi.org/10.1161/01.RES.0000258116.60404.ad.
Vahebi S, Ota A, Li M, Warren CM, de Tombe PP, Wang Y, et al. p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity. Circ Res. 2007 Feb 16;100(3):408–15.
Vahebi, Susan, et al. “p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity.Circ Res, vol. 100, no. 3, Feb. 2007, pp. 408–15. Pubmed, doi:10.1161/01.RES.0000258116.60404.ad.
Vahebi S, Ota A, Li M, Warren CM, de Tombe PP, Wang Y, Solaro RJ. p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity. Circ Res. 2007 Feb 16;100(3):408–415.

Published In

Circ Res

DOI

EISSN

1524-4571

Publication Date

February 16, 2007

Volume

100

Issue

3

Start / End Page

408 / 415

Location

United States

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Troponin I
  • Tropomyosin
  • Transgenes
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Sarcomeres
  • Recombinant Fusion Proteins
  • Protein Transport
  • Protein Processing, Post-Translational