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Nitric oxide donors protect murine myocardium against infarction via modulation of mitochondrial permeability transition.

Publication ,  Journal Article
Wang, G; Liem, DA; Vondriska, TM; Honda, HM; Korge, P; Pantaleon, DM; Qiao, X; Wang, Y; Weiss, JN; Ping, P
Published in: Am J Physiol Heart Circ Physiol
March 2005

Mitochondrial permeability transition (MPT) pores have recently been implicated as a potential mediator of myocardial ischemic injury. Nitric oxide (NO) donors induce a powerful late phase of cardioprotection against ischemia-reperfusion injury; however, the cellular mechanisms involved are poorly understood. The role of MPT pores as a target of cardioprotective signaling pathways activated by NO has never been explored in detail. Thus mice were administered the NO donor diethylenetriamine (DETA)/NO (4 doses of 0.1 mg/kg i.v. each) 24 h before 30 min of coronary artery occlusion followed by 24 h of reperfusion. Infarct size was significantly reduced in DETA/NO-treated mice (30 +/- 2% of risk region in treated mice vs. 50 +/- 2% in control mice; P < 0.05), which demonstrates powerful cardioprotection. To examine the role of MPT pores, mice were administered atractyloside (Atr; 25 mg/kg i.v.), which induces adenine nucleotide translocase-dependent MPT, 20 min before ischemia. Atr blocked the infarct-sparing effects of DETA/NO (infarct size, 58 +/- 1 vs. 30 +/- 2% of risk region in DETA/NO; P < 0.05), whereas Atr alone had no effect. Mitochondria isolated from DETA/NO-treated mice exhibited increased resistance to Ca(2+)-induced swelling by 20 micromol/l CaCl(2) or by the higher concentration of 200 micromol/l, which suggests that cardioprotection involves decreased propensity for MPT. Preincubation of mitochondria from control hearts with 30 nmol/l of the pore inhibitor cyclosporin A prevented swelling by 200 micromol/l CaCl(2), thereby confirming that Ca(2+) induces mitochondrial swelling via MPT. In accordance with the effects on infarct size, administration of Atr to the mice significantly abrogated DETA/NO-induced protection against Ca(2+)-induced mitochondrial swelling. These phenotypic alterations were associated with an increase in the antiapoptotic protein Bcl-2, which suggests that the underlying mechanisms may involve inhibition of cell death by Bcl-2. These data suggest that a critical process during NO donor-induced cardioprotection is to prevent MPT pore opening potentially via targeting of the adenine nucleotide translocator.

Duke Scholars

Published In

Am J Physiol Heart Circ Physiol

DOI

ISSN

0363-6135

Publication Date

March 2005

Volume

288

Issue

3

Start / End Page

H1290 / H1295

Location

United States

Related Subject Headings

  • Up-Regulation
  • Proto-Oncogene Proteins c-bcl-2
  • Polyamines
  • Nitric Oxide Donors
  • Nitric Oxide
  • Myocardium
  • Myocardial Infarction
  • Mitochondrial Swelling
  • Mitochondria
  • Mice, Inbred ICR
 

Citation

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Wang, G., Liem, D. A., Vondriska, T. M., Honda, H. M., Korge, P., Pantaleon, D. M., … Ping, P. (2005). Nitric oxide donors protect murine myocardium against infarction via modulation of mitochondrial permeability transition. Am J Physiol Heart Circ Physiol, 288(3), H1290–H1295. https://doi.org/10.1152/ajpheart.00796.2004
Wang, Guangwu, David A. Liem, Thomas M. Vondriska, Henry M. Honda, Paavo Korge, Dawn M. Pantaleon, Xin Qiao, Yibin Wang, James N. Weiss, and Peipei Ping. “Nitric oxide donors protect murine myocardium against infarction via modulation of mitochondrial permeability transition.Am J Physiol Heart Circ Physiol 288, no. 3 (March 2005): H1290–95. https://doi.org/10.1152/ajpheart.00796.2004.
Wang G, Liem DA, Vondriska TM, Honda HM, Korge P, Pantaleon DM, et al. Nitric oxide donors protect murine myocardium against infarction via modulation of mitochondrial permeability transition. Am J Physiol Heart Circ Physiol. 2005 Mar;288(3):H1290–5.
Wang, Guangwu, et al. “Nitric oxide donors protect murine myocardium against infarction via modulation of mitochondrial permeability transition.Am J Physiol Heart Circ Physiol, vol. 288, no. 3, Mar. 2005, pp. H1290–95. Pubmed, doi:10.1152/ajpheart.00796.2004.
Wang G, Liem DA, Vondriska TM, Honda HM, Korge P, Pantaleon DM, Qiao X, Wang Y, Weiss JN, Ping P. Nitric oxide donors protect murine myocardium against infarction via modulation of mitochondrial permeability transition. Am J Physiol Heart Circ Physiol. 2005 Mar;288(3):H1290–H1295.

Published In

Am J Physiol Heart Circ Physiol

DOI

ISSN

0363-6135

Publication Date

March 2005

Volume

288

Issue

3

Start / End Page

H1290 / H1295

Location

United States

Related Subject Headings

  • Up-Regulation
  • Proto-Oncogene Proteins c-bcl-2
  • Polyamines
  • Nitric Oxide Donors
  • Nitric Oxide
  • Myocardium
  • Myocardial Infarction
  • Mitochondrial Swelling
  • Mitochondria
  • Mice, Inbred ICR