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An increase in the myocardial PCr/ATP ratio in GLUT4 null mice.

Publication ,  Journal Article
Weiss, RG; Chatham, JC; Georgakopolous, D; Charron, MJ; Wallimann, T; Kay, L; Walzel, B; Wang, Y; Kass, DA; Gerstenblith, G; Chacko, VP
Published in: FASEB J
April 2002

ATP and creatine phosphate (PCr) are prime myocardial high-energy phosphates. Their relative concentrations are conserved among mammalian species and across a range of physiologic cardiac workloads. The cardiac PCr/ATP ratio is decreased with several pathologic conditions, such as ischemia and heart failure, but there are no reports of an increase in the cardiac PCr/ATP ratio in any species or with interventions. We studied the in vivo energetics in transgenic mice lacking expression of the glucose transport protein GLUT4 (G4N) and observed a significant 60% increase in the myocardial PCr/ATP ratio in G4N that was confirmed in three different experimental settings including intact animals. The higher PCr/ATP in G4N is cardiac-specific and is due to higher total cardiac creatine (CR) concentrations in G4N than in wild-type (WT). However, [ATP], [ADP], and -DG(-ATP) did not differ between the strains. Expression of the creatine transport protein (CreaT) that is responsible for creatine uptake in myocytes was preserved in G4N cardiac tissue. These observations demonstrate, for the first time to our knowledge, that G4N manifest a unique increase in the cardiac PCr/ATP ratio, which suggests a novel genetic strategy for increasing myocardial creatine levels.

Duke Scholars

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

April 2002

Volume

16

Issue

6

Start / End Page

613 / 615

Location

United States

Related Subject Headings

  • Systole
  • Phosphocreatine
  • Organ Specificity
  • Myocardium
  • Muscle Proteins
  • Monosaccharide Transport Proteins
  • Models, Cardiovascular
  • Mice, Transgenic
  • Mice, Knockout
  • Mice
 

Citation

APA
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Weiss, R. G., Chatham, J. C., Georgakopolous, D., Charron, M. J., Wallimann, T., Kay, L., … Chacko, V. P. (2002). An increase in the myocardial PCr/ATP ratio in GLUT4 null mice. FASEB J, 16(6), 613–615. https://doi.org/10.1096/fj.01-0462fje
Weiss, Robert G., John C. Chatham, Dimitrios Georgakopolous, Maureen J. Charron, Theo Wallimann, Laurence Kay, Bernd Walzel, et al. “An increase in the myocardial PCr/ATP ratio in GLUT4 null mice.FASEB J 16, no. 6 (April 2002): 613–15. https://doi.org/10.1096/fj.01-0462fje.
Weiss RG, Chatham JC, Georgakopolous D, Charron MJ, Wallimann T, Kay L, et al. An increase in the myocardial PCr/ATP ratio in GLUT4 null mice. FASEB J. 2002 Apr;16(6):613–5.
Weiss, Robert G., et al. “An increase in the myocardial PCr/ATP ratio in GLUT4 null mice.FASEB J, vol. 16, no. 6, Apr. 2002, pp. 613–15. Pubmed, doi:10.1096/fj.01-0462fje.
Weiss RG, Chatham JC, Georgakopolous D, Charron MJ, Wallimann T, Kay L, Walzel B, Wang Y, Kass DA, Gerstenblith G, Chacko VP. An increase in the myocardial PCr/ATP ratio in GLUT4 null mice. FASEB J. 2002 Apr;16(6):613–615.

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

April 2002

Volume

16

Issue

6

Start / End Page

613 / 615

Location

United States

Related Subject Headings

  • Systole
  • Phosphocreatine
  • Organ Specificity
  • Myocardium
  • Muscle Proteins
  • Monosaccharide Transport Proteins
  • Models, Cardiovascular
  • Mice, Transgenic
  • Mice, Knockout
  • Mice