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Topical application of viral vectors for epidermal gene transfer.

Publication ,  Journal Article
Lu, B; Federoff, HJ; Wang, Y; Goldsmith, LA; Scott, G
Published in: J Invest Dermatol
May 1997

Efficient gene transfer with extended gene expression is essential for successful treatment of skin diseases using gene therapy. Previously we evaluated a physical gene transfer method (gene gun delivery) for its ability to transfect the epidermis in vivo. In this study, we tested two viral vectors for their ability to transduce murine epidermis through topical application. Both an adenoviral vector and a herpes simplex virus (HSV) amplicon vector transduced murine epidermis with high efficiency after topical application. Differences in amount and duration of transgene expression were compared between these two vectors. Quantitative analysis of reporter lacZ gene expression showed that the viral vector-mediated gene transfers were superior to gene-gun delivery of plasmid DNA. Significant necrosis and cytotoxicity, however, were observed in the HSV-treated skin. In addition, we show that murine epidermis developed hyperkeratosis and acanthosis 4 d after an adenoviral vector containing a human TGF-alpha expression unit was applied topically. Finally we demonstrate the feasibility of transduction of fetal skin in utero by intraamniotic injection of an adenovirus vector.

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Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

May 1997

Volume

108

Issue

5

Start / End Page

803 / 808

Location

United States

Related Subject Headings

  • Skin Diseases
  • Skin
  • Mice
  • Injections
  • Hyperkeratosis, Epidermolytic
  • Herpesviridae
  • Genetic Therapy
  • Genes, Viral
  • Genes, Reporter
  • Gene Transfer Techniques
 

Citation

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Lu, B., Federoff, H. J., Wang, Y., Goldsmith, L. A., & Scott, G. (1997). Topical application of viral vectors for epidermal gene transfer. J Invest Dermatol, 108(5), 803–808. https://doi.org/10.1111/1523-1747.ep12292254
Lu, B., H. J. Federoff, Y. Wang, L. A. Goldsmith, and G. Scott. “Topical application of viral vectors for epidermal gene transfer.J Invest Dermatol 108, no. 5 (May 1997): 803–8. https://doi.org/10.1111/1523-1747.ep12292254.
Lu B, Federoff HJ, Wang Y, Goldsmith LA, Scott G. Topical application of viral vectors for epidermal gene transfer. J Invest Dermatol. 1997 May;108(5):803–8.
Lu, B., et al. “Topical application of viral vectors for epidermal gene transfer.J Invest Dermatol, vol. 108, no. 5, May 1997, pp. 803–08. Pubmed, doi:10.1111/1523-1747.ep12292254.
Lu B, Federoff HJ, Wang Y, Goldsmith LA, Scott G. Topical application of viral vectors for epidermal gene transfer. J Invest Dermatol. 1997 May;108(5):803–808.
Journal cover image

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

May 1997

Volume

108

Issue

5

Start / End Page

803 / 808

Location

United States

Related Subject Headings

  • Skin Diseases
  • Skin
  • Mice
  • Injections
  • Hyperkeratosis, Epidermolytic
  • Herpesviridae
  • Genetic Therapy
  • Genes, Viral
  • Genes, Reporter
  • Gene Transfer Techniques