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Cholesterol-induced macrophage apoptosis requires ER stress pathways and engagement of the type A scavenger receptor.

Publication ,  Journal Article
Devries-Seimon, T; Li, Y; Yao, PM; Stone, E; Wang, Y; Davis, RJ; Flavell, R; Tabas, I
Published in: J Cell Biol
October 10, 2005

Macrophage death in advanced atherosclerosis promotes necrosis and plaque destabilization. A likely cause of macrophage death is accumulation of free cholesterol (FC) in the ER, leading to activation of the unfolded protein response (UPR) and C/EBP homologous protein (CHOP)-induced apoptosis. Here we show that p38 MAPK signaling is necessary for CHOP induction and apoptosis. Additionally, two other signaling pathways must cooperate with p38-CHOP to effect apoptosis. One involves the type A scavenger receptor (SRA). As evidence, FC loading by non-SRA mechanisms activates p38 and CHOP, but not apoptosis unless the SRA is engaged. The other pathway involves c-Jun NH2-terminal kinase (JNK)2, which is activated by cholesterol trafficking to the ER, but is independent of CHOP. Thus, FC-induced apoptosis requires cholesterol trafficking to the ER, which triggers p38-CHOP and JNK2, and engagement of the SRA. These findings have important implications for understanding how the UPR, MAPKs, and the SRA might conspire to cause macrophage death, lesional necrosis, and plaque destabilization in advanced atherosclerotic lesions.

Duke Scholars

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

October 10, 2005

Volume

171

Issue

1

Start / End Page

61 / 73

Location

United States

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Signal Transduction
  • Scavenger Receptors, Class A
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Protein Conformation
  • Phosphorylation
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
 

Citation

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Devries-Seimon, T., Li, Y., Yao, P. M., Stone, E., Wang, Y., Davis, R. J., … Tabas, I. (2005). Cholesterol-induced macrophage apoptosis requires ER stress pathways and engagement of the type A scavenger receptor. J Cell Biol, 171(1), 61–73. https://doi.org/10.1083/jcb.200502078
Devries-Seimon, Tracie, Yankun Li, Pin Mei Yao, Elizabeth Stone, Yibin Wang, Roger J. Davis, Richard Flavell, and Ira Tabas. “Cholesterol-induced macrophage apoptosis requires ER stress pathways and engagement of the type A scavenger receptor.J Cell Biol 171, no. 1 (October 10, 2005): 61–73. https://doi.org/10.1083/jcb.200502078.
Devries-Seimon T, Li Y, Yao PM, Stone E, Wang Y, Davis RJ, et al. Cholesterol-induced macrophage apoptosis requires ER stress pathways and engagement of the type A scavenger receptor. J Cell Biol. 2005 Oct 10;171(1):61–73.
Devries-Seimon, Tracie, et al. “Cholesterol-induced macrophage apoptosis requires ER stress pathways and engagement of the type A scavenger receptor.J Cell Biol, vol. 171, no. 1, Oct. 2005, pp. 61–73. Pubmed, doi:10.1083/jcb.200502078.
Devries-Seimon T, Li Y, Yao PM, Stone E, Wang Y, Davis RJ, Flavell R, Tabas I. Cholesterol-induced macrophage apoptosis requires ER stress pathways and engagement of the type A scavenger receptor. J Cell Biol. 2005 Oct 10;171(1):61–73.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

October 10, 2005

Volume

171

Issue

1

Start / End Page

61 / 73

Location

United States

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Signal Transduction
  • Scavenger Receptors, Class A
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Protein Conformation
  • Phosphorylation
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice