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Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma.

Publication ,  Journal Article
Grasemann, C; Gratias, S; Stephan, H; Schüler, A; Schramm, A; Klein-Hitpass, L; Rieder, H; Schneider, S; Kappes, F; Eggert, A; Lohmann, DR
Published in: Oncogene
September 2005

The paediatric eye tumour retinoblastoma is initiated by inactivation of RB1, a tumour suppressor on chromosome 13q. In addition to RB1 loss, many retinoblastomas show other genetic alterations including gains on chromosomes 6p21-pter and 1q31-q32. Recently, the minimal region of gains on chromosome 6 was narrowed to band p22. We examined genomic gains and expression changes in primary retinoblastomas to identify potential target genes in 6p22. Quantitative multiplex PCR detected copy numbers > or = 3 in 25 (33%) tumours and no gains in 31 of 76 (40%) tumours. The remaining 20 (26%) samples showed gains only at some loci, most often including E2F3 and DEK in 6p22.3. Analysis of RNA from 21 primary retinoblastomas showed that expression levels of these and some other genes in 6p22 correspond to DNA gains. However, KIF 13A, a reported candidate oncogene on 6p, was expressed at low levels or absent. Clinical manifestation of tumours with gains at all 6p22 loci was distinct in that distribution of age at diagnosis was markedly shifted to older age compared to tumours with no or partial gains. In summary, our results suggest that DEK and E2F3 are potential targets of 6p gains in retinoblastoma.

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Published In

Oncogene

DOI

EISSN

1476-5594

ISSN

0950-9232

Publication Date

September 2005

Volume

24

Issue

42

Start / End Page

6441 / 6449

Related Subject Headings

  • Transcription Factors
  • Retinoblastoma
  • Retinal Neoplasms
  • Polymerase Chain Reaction
  • Poly-ADP-Ribose Binding Proteins
  • Oncology & Carcinogenesis
  • Oncogene Proteins
  • Humans
  • Gene Expression
  • E2F3 Transcription Factor
 

Citation

APA
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ICMJE
MLA
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Grasemann, C., Gratias, S., Stephan, H., Schüler, A., Schramm, A., Klein-Hitpass, L., … Lohmann, D. R. (2005). Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma. Oncogene, 24(42), 6441–6449. https://doi.org/10.1038/sj.onc.1208792
Grasemann, Corinna, Sandrine Gratias, Harald Stephan, Andreas Schüler, Alexander Schramm, Ludger Klein-Hitpass, Harald Rieder, et al. “Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma.Oncogene 24, no. 42 (September 2005): 6441–49. https://doi.org/10.1038/sj.onc.1208792.
Grasemann C, Gratias S, Stephan H, Schüler A, Schramm A, Klein-Hitpass L, et al. Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma. Oncogene. 2005 Sep;24(42):6441–9.
Grasemann, Corinna, et al. “Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma.Oncogene, vol. 24, no. 42, Sept. 2005, pp. 6441–49. Epmc, doi:10.1038/sj.onc.1208792.
Grasemann C, Gratias S, Stephan H, Schüler A, Schramm A, Klein-Hitpass L, Rieder H, Schneider S, Kappes F, Eggert A, Lohmann DR. Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma. Oncogene. 2005 Sep;24(42):6441–6449.

Published In

Oncogene

DOI

EISSN

1476-5594

ISSN

0950-9232

Publication Date

September 2005

Volume

24

Issue

42

Start / End Page

6441 / 6449

Related Subject Headings

  • Transcription Factors
  • Retinoblastoma
  • Retinal Neoplasms
  • Polymerase Chain Reaction
  • Poly-ADP-Ribose Binding Proteins
  • Oncology & Carcinogenesis
  • Oncogene Proteins
  • Humans
  • Gene Expression
  • E2F3 Transcription Factor