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Matrix Stiffness Modulates Patient-Derived Glioblastoma Cell Fates in Three-Dimensional Hydrogels.

Publication ,  Journal Article
Wang, C; Sinha, S; Jiang, X; Murphy, L; Fitch, S; Wilson, C; Grant, G; Yang, F
Published in: Tissue Eng Part A
March 2021

Cancer progression is known to be accompanied by changes in tissue stiffness. Previous studies have primarily employed immortalized cell lines and 2D hydrogel substrates, which do not recapitulate the 3D tumor niche. How matrix stiffness affects patient-derived cancer cell fate in 3D remains unclear. In this study, we report a matrix metalloproteinase-degradable poly(ethylene-glycol)-based hydrogel platform with brain-mimicking biochemical cues and tunable stiffness (40-26,600 Pa) for 3D culture of patient-derived glioblastoma xenograft (PDTX GBM) cells. Our results demonstrate that decreasing hydrogel stiffness enhanced PDTX GBM cell proliferation, and hydrogels with stiffness 240 Pa and below supported robust PDTX GBM cell spreading in 3D. PDTX GBM cells encapsulated in hydrogels demonstrated higher drug resistance than 2D control, and increasing hydrogel stiffness further enhanced drug resistance. Such 3D hydrogel platforms may provide a valuable tool for mechanistic studies of the role of niche cues in modulating cancer progression for different cancer types.

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Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

March 2021

Volume

27

Issue

5-6

Start / End Page

390 / 401

Location

United States

Related Subject Headings

  • Hydrogels
  • Humans
  • Glioblastoma
  • Cell Proliferation
  • Cell Line, Tumor
  • Cell Differentiation
  • Brain Neoplasms
  • Biomedical Engineering
  • 4003 Biomedical engineering
  • 0912 Materials Engineering
 

Citation

APA
Chicago
ICMJE
MLA
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Wang, C., Sinha, S., Jiang, X., Murphy, L., Fitch, S., Wilson, C., … Yang, F. (2021). Matrix Stiffness Modulates Patient-Derived Glioblastoma Cell Fates in Three-Dimensional Hydrogels. Tissue Eng Part A, 27(5–6), 390–401. https://doi.org/10.1089/ten.TEA.2020.0110
Wang, Christine, Sauradeep Sinha, Xinyi Jiang, Luke Murphy, Sergio Fitch, Christy Wilson, Gerald Grant, and Fan Yang. “Matrix Stiffness Modulates Patient-Derived Glioblastoma Cell Fates in Three-Dimensional Hydrogels.Tissue Eng Part A 27, no. 5–6 (March 2021): 390–401. https://doi.org/10.1089/ten.TEA.2020.0110.
Wang C, Sinha S, Jiang X, Murphy L, Fitch S, Wilson C, et al. Matrix Stiffness Modulates Patient-Derived Glioblastoma Cell Fates in Three-Dimensional Hydrogels. Tissue Eng Part A. 2021 Mar;27(5–6):390–401.
Wang, Christine, et al. “Matrix Stiffness Modulates Patient-Derived Glioblastoma Cell Fates in Three-Dimensional Hydrogels.Tissue Eng Part A, vol. 27, no. 5–6, Mar. 2021, pp. 390–401. Pubmed, doi:10.1089/ten.TEA.2020.0110.
Wang C, Sinha S, Jiang X, Murphy L, Fitch S, Wilson C, Grant G, Yang F. Matrix Stiffness Modulates Patient-Derived Glioblastoma Cell Fates in Three-Dimensional Hydrogels. Tissue Eng Part A. 2021 Mar;27(5–6):390–401.

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

March 2021

Volume

27

Issue

5-6

Start / End Page

390 / 401

Location

United States

Related Subject Headings

  • Hydrogels
  • Humans
  • Glioblastoma
  • Cell Proliferation
  • Cell Line, Tumor
  • Cell Differentiation
  • Brain Neoplasms
  • Biomedical Engineering
  • 4003 Biomedical engineering
  • 0912 Materials Engineering