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Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging.

Publication ,  Journal Article
Yecies, D; Liba, O; SoRelle, ED; Dutta, R; Yuan, E; Vogel, H; Grant, GA; de la Zerda, A
Published in: Sci Rep
July 17, 2019

Current in vivo neuroimaging techniques provide limited field of view or spatial resolution and often require exogenous contrast. These limitations prohibit detailed structural imaging across wide fields of view and hinder intraoperative tumor margin detection. Here we present a novel neuroimaging technique, speckle-modulating optical coherence tomography (SM-OCT), which allows us to image the brains of live mice and ex vivo human samples with unprecedented resolution and wide field of view using only endogenous contrast. The increased visibility provided by speckle elimination reveals white matter fascicles and cortical layer architecture in brains of live mice. To our knowledge, the data reported herein represents the highest resolution imaging of murine white matter structure achieved in vivo across a wide field of view of several millimeters. When applied to an orthotopic murine glioblastoma xenograft model, SM-OCT readily identifies brain tumor margins with resolution of approximately 10 μm. SM-OCT of ex vivo human temporal lobe tissue reveals fine structures including cortical layers and myelinated axons. Finally, when applied to an ex vivo sample of a low-grade glioma resection margin, SM-OCT is able to resolve the brain tumor margin. Based on these findings, SM-OCT represents a novel approach for intraoperative tumor margin detection and in vivo neuroimaging.

Duke Scholars

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

July 17, 2019

Volume

9

Issue

1

Start / End Page

10388

Location

England

Related Subject Headings

  • White Matter
  • Tomography, Optical Coherence
  • Neuroimaging
  • Mice, Nude
  • Mice, Inbred C57BL
  • Mice
  • Margins of Excision
  • Humans
  • Glioma
  • Glioblastoma
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yecies, D., Liba, O., SoRelle, E. D., Dutta, R., Yuan, E., Vogel, H., … de la Zerda, A. (2019). Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging. Sci Rep, 9(1), 10388. https://doi.org/10.1038/s41598-019-45902-9
Yecies, Derek, Orly Liba, Elliott D. SoRelle, Rebecca Dutta, Edwin Yuan, Hannes Vogel, Gerald A. Grant, and Adam de la Zerda. “Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging.Sci Rep 9, no. 1 (July 17, 2019): 10388. https://doi.org/10.1038/s41598-019-45902-9.
Yecies D, Liba O, SoRelle ED, Dutta R, Yuan E, Vogel H, et al. Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging. Sci Rep. 2019 Jul 17;9(1):10388.
Yecies, Derek, et al. “Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging.Sci Rep, vol. 9, no. 1, July 2019, p. 10388. Pubmed, doi:10.1038/s41598-019-45902-9.
Yecies D, Liba O, SoRelle ED, Dutta R, Yuan E, Vogel H, Grant GA, de la Zerda A. Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging. Sci Rep. 2019 Jul 17;9(1):10388.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

July 17, 2019

Volume

9

Issue

1

Start / End Page

10388

Location

England

Related Subject Headings

  • White Matter
  • Tomography, Optical Coherence
  • Neuroimaging
  • Mice, Nude
  • Mice, Inbred C57BL
  • Mice
  • Margins of Excision
  • Humans
  • Glioma
  • Glioblastoma