An engineered ScCas9 with broad PAM range and high specificity and activity.
Publication
, Journal Article
Chatterjee, P; Jakimo, N; Lee, J; Amrani, N; Rodríguez, T; Koseki, SRT; Tysinger, E; Qing, R; Hao, S; Sontheimer, EJ; Jacobson, J
Published in: Nature biotechnology
October 2020
CRISPR enzymes require a protospacer-adjacent motif (PAM) near the target cleavage site, constraining the sequences accessible for editing. In the present study, we combine protein motifs from several orthologs to engineer two variants of Streptococcus canis Cas9-Sc++ and a higher-fidelity mutant HiFi-Sc++-that have simultaneously broad 5'-NNG-3' PAM compatibility, robust DNA-cleavage activity and minimal off-target activity. Sc++ and HiFi-Sc++ extend the use of CRISPR editing for diverse applications.
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Published In
Nature biotechnology
DOI
EISSN
1546-1696
ISSN
1087-0156
Publication Date
October 2020
Volume
38
Issue
10
Start / End Page
1154 / 1158
Related Subject Headings
- Streptococcus
- Gene Editing
- DNA Cleavage
- Clustered Regularly Interspaced Short Palindromic Repeats
- CRISPR-Cas Systems
- CRISPR-Associated Protein 9
- Amino Acid Motifs
Citation
APA
Chicago
ICMJE
MLA
NLM
Chatterjee, P., Jakimo, N., Lee, J., Amrani, N., Rodríguez, T., Koseki, S. R. T., … Jacobson, J. (2020). An engineered ScCas9 with broad PAM range and high specificity and activity. Nature Biotechnology, 38(10), 1154–1158. https://doi.org/10.1038/s41587-020-0517-0
Chatterjee, Pranam, Noah Jakimo, Jooyoung Lee, Nadia Amrani, Tomás Rodríguez, Sabrina R. T. Koseki, Emma Tysinger, et al. “An engineered ScCas9 with broad PAM range and high specificity and activity.” Nature Biotechnology 38, no. 10 (October 2020): 1154–58. https://doi.org/10.1038/s41587-020-0517-0.
Chatterjee P, Jakimo N, Lee J, Amrani N, Rodríguez T, Koseki SRT, et al. An engineered ScCas9 with broad PAM range and high specificity and activity. Nature biotechnology. 2020 Oct;38(10):1154–8.
Chatterjee, Pranam, et al. “An engineered ScCas9 with broad PAM range and high specificity and activity.” Nature Biotechnology, vol. 38, no. 10, Oct. 2020, pp. 1154–58. Epmc, doi:10.1038/s41587-020-0517-0.
Chatterjee P, Jakimo N, Lee J, Amrani N, Rodríguez T, Koseki SRT, Tysinger E, Qing R, Hao S, Sontheimer EJ, Jacobson J. An engineered ScCas9 with broad PAM range and high specificity and activity. Nature biotechnology. 2020 Oct;38(10):1154–1158.
Published In
Nature biotechnology
DOI
EISSN
1546-1696
ISSN
1087-0156
Publication Date
October 2020
Volume
38
Issue
10
Start / End Page
1154 / 1158
Related Subject Headings
- Streptococcus
- Gene Editing
- DNA Cleavage
- Clustered Regularly Interspaced Short Palindromic Repeats
- CRISPR-Cas Systems
- CRISPR-Associated Protein 9
- Amino Acid Motifs