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Dendritic cells stimulated with Actinobacillus actinomycetemcomitans elicit rapid gamma interferon responses by natural killer cells.

Publication ,  Journal Article
Kikuchi, T; Hahn, CL; Tanaka, S; Barbour, SE; Schenkein, HA; Tew, JG
Published in: Infect Immun
September 2004

Human immunoglobulin G2 (IgG2) responses are gamma interferon (IFN-gamma) dependent, and monocyte-derived dendritic cells (mDCs) promote IgG2 production. DCs spontaneously emerge from monocytes in cultures prepared from localized aggressive periodontitis (LagP) patients, and these patients have high levels of IgG2 that is reactive with Actinobacillus actinomycetemcomitans. These results prompted the hypothesis that an interaction between mDCs and A. actinomycetemcomitans promotes IFN-gamma production, and IFN-gamma is known to promote both immunopathology and protective IgG2. A. actinomycetemcomitans induced mDCs to produce interleukin-12 (IL-12), and the addition of A. actinomycetemcomitans and DCs to cultured peripheral blood lymphocytes elicited high levels of IFN-gamma within just 24 h. In contrast, IL-4 was not detectable although DC-derived IL-10 production was apparent. A. actinomycetemcomitans-stimulated macrophages prepared from the same monocytes lacked the ability to induce IL-12 or IFN-gamma responses. NK cells of the innate immune system were the primary source of this early IFN-gamma, although CD8 T cells also contributed some. The NK cell-derived IFN-gamma was IL-12 dependent, and A. actinomycetemcomitans-DC interactions were Toll-like receptor 4 dependent. A. actinomycetemcomitans and A. actinomycetemcomitans lipopolysaccharide (LPS) were more potent than Escherichia coli and E. coli LPS in the ability to induce DC IL-12 and IFN-gamma. The ability of A. actinomycetemcomitans-stimulated DCs to induce NK cells to rapidly produce IFN-gamma in the absence of detectable IL-4 suggests their potential for skewing responses toward Th1. This may help explain the presence of Th1-associated cytokines in gingival crevicular fluid (GCF) from LagP patients and the high levels of IgG2 in their serum and GCF that is reactive with A. actinomycetemcomitans.

Duke Scholars

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

September 2004

Volume

72

Issue

9

Start / End Page

5089 / 5096

Location

United States

Related Subject Headings

  • Toll-Like Receptors
  • Toll-Like Receptor 4
  • Receptors, Cell Surface
  • Protein Subunits
  • Monocytes
  • Microbiology
  • Membrane Glycoproteins
  • Lipopolysaccharides
  • Killer Cells, Natural
  • Interleukin-12
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kikuchi, T., Hahn, C. L., Tanaka, S., Barbour, S. E., Schenkein, H. A., & Tew, J. G. (2004). Dendritic cells stimulated with Actinobacillus actinomycetemcomitans elicit rapid gamma interferon responses by natural killer cells. Infect Immun, 72(9), 5089–5096. https://doi.org/10.1128/IAI.72.9.5089-5096.2004
Kikuchi, T., C. L. Hahn, S. Tanaka, S. E. Barbour, H. A. Schenkein, and J. G. Tew. “Dendritic cells stimulated with Actinobacillus actinomycetemcomitans elicit rapid gamma interferon responses by natural killer cells.Infect Immun 72, no. 9 (September 2004): 5089–96. https://doi.org/10.1128/IAI.72.9.5089-5096.2004.
Kikuchi T, Hahn CL, Tanaka S, Barbour SE, Schenkein HA, Tew JG. Dendritic cells stimulated with Actinobacillus actinomycetemcomitans elicit rapid gamma interferon responses by natural killer cells. Infect Immun. 2004 Sep;72(9):5089–96.
Kikuchi, T., et al. “Dendritic cells stimulated with Actinobacillus actinomycetemcomitans elicit rapid gamma interferon responses by natural killer cells.Infect Immun, vol. 72, no. 9, Sept. 2004, pp. 5089–96. Pubmed, doi:10.1128/IAI.72.9.5089-5096.2004.
Kikuchi T, Hahn CL, Tanaka S, Barbour SE, Schenkein HA, Tew JG. Dendritic cells stimulated with Actinobacillus actinomycetemcomitans elicit rapid gamma interferon responses by natural killer cells. Infect Immun. 2004 Sep;72(9):5089–5096.

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

September 2004

Volume

72

Issue

9

Start / End Page

5089 / 5096

Location

United States

Related Subject Headings

  • Toll-Like Receptors
  • Toll-Like Receptor 4
  • Receptors, Cell Surface
  • Protein Subunits
  • Monocytes
  • Microbiology
  • Membrane Glycoproteins
  • Lipopolysaccharides
  • Killer Cells, Natural
  • Interleukin-12