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Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer.

Publication ,  Journal Article
Puram, SV; Tirosh, I; Parikh, AS; Patel, AP; Yizhak, K; Gillespie, S; Rodman, C; Luo, CL; Mroz, EA; Emerick, KS; Deschler, DG; Varvares, MA ...
Published in: Cell
December 14, 2017

The diverse malignant, stromal, and immune cells in tumors affect growth, metastasis, and response to therapy. We profiled transcriptomes of ∼6,000 single cells from 18 head and neck squamous cell carcinoma (HNSCC) patients, including five matched pairs of primary tumors and lymph node metastases. Stromal and immune cells had consistent expression programs across patients. Conversely, malignant cells varied within and between tumors in their expression of signatures related to cell cycle, stress, hypoxia, epithelial differentiation, and partial epithelial-to-mesenchymal transition (p-EMT). Cells expressing the p-EMT program spatially localized to the leading edge of primary tumors. By integrating single-cell transcriptomes with bulk expression profiles for hundreds of tumors, we refined HNSCC subtypes by their malignant and stromal composition and established p-EMT as an independent predictor of nodal metastasis, grade, and adverse pathologic features. Our results provide insight into the HNSCC ecosystem and define stromal interactions and a p-EMT program associated with metastasis.

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Published In

Cell

DOI

EISSN

1097-4172

Publication Date

December 14, 2017

Volume

171

Issue

7

Start / End Page

1611 / 1624.e24

Location

United States

Related Subject Headings

  • Tumor Microenvironment
  • Single-Cell Analysis
  • Neoplasm Metastasis
  • Male
  • Humans
  • Head and Neck Neoplasms
  • Gene Expression Profiling
  • Epithelial-Mesenchymal Transition
  • Developmental Biology
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Puram, S. V., Tirosh, I., Parikh, A. S., Patel, A. P., Yizhak, K., Gillespie, S., … Bernstein, B. E. (2017). Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell, 171(7), 1611-1624.e24. https://doi.org/10.1016/j.cell.2017.10.044
Puram, Sidharth V., Itay Tirosh, Anuraag S. Parikh, Anoop P. Patel, Keren Yizhak, Shawn Gillespie, Christopher Rodman, et al. “Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer.Cell 171, no. 7 (December 14, 2017): 1611-1624.e24. https://doi.org/10.1016/j.cell.2017.10.044.
Puram SV, Tirosh I, Parikh AS, Patel AP, Yizhak K, Gillespie S, et al. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell. 2017 Dec 14;171(7):1611-1624.e24.
Puram, Sidharth V., et al. “Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer.Cell, vol. 171, no. 7, Dec. 2017, pp. 1611-1624.e24. Pubmed, doi:10.1016/j.cell.2017.10.044.
Puram SV, Tirosh I, Parikh AS, Patel AP, Yizhak K, Gillespie S, Rodman C, Luo CL, Mroz EA, Emerick KS, Deschler DG, Varvares MA, Mylvaganam R, Rozenblatt-Rosen O, Rocco JW, Faquin WC, Lin DT, Regev A, Bernstein BE. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell. 2017 Dec 14;171(7):1611-1624.e24.
Journal cover image

Published In

Cell

DOI

EISSN

1097-4172

Publication Date

December 14, 2017

Volume

171

Issue

7

Start / End Page

1611 / 1624.e24

Location

United States

Related Subject Headings

  • Tumor Microenvironment
  • Single-Cell Analysis
  • Neoplasm Metastasis
  • Male
  • Humans
  • Head and Neck Neoplasms
  • Gene Expression Profiling
  • Epithelial-Mesenchymal Transition
  • Developmental Biology
  • Cells, Cultured