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The proto-oncogene bcl-3 encodes an I kappa B protein.

Publication ,  Journal Article
Kerr, LD; Duckett, CS; Wamsley, P; Zhang, Q; Chiao, P; Nabel, G; McKeithan, TW; Baeuerle, PA; Verma, IM
Published in: Genes Dev
December 1992

The bcl-3 gene product, overexpressed in chronic lymphocytic leukemia (CLL) patients with the translocation t(14;19), is a member of the I kappa B family. The bcl-3 protein is able to inhibit the DNA binding and trans-activation of authentic NF-kappa B heterodimers p50-p65 and p49-p65, as well as p50 and p49 homodimers. The bcl-3 protein does not inhibit either the DNA-binding activity of the Rel protein or its ability to trans-activate genes linked to the kappa B site. A human 37-kD protein (I kappa B alpha), identified previously as a member of the I kappa B family, is also unable to inhibit DNA-binding activity of the Rel protein. However, unlike bcl-3, the 37-kD (I kappa B alpha) protein has no effect on the DNA-binding activity of p50 or p49 homodimers. Two dimensional phosphotryptic peptide maps of the human bcl-3 and the human 37-kD (I kappa B alpha) proteins reveal that the phosphopeptides from the 37-kD (I kappa B alpha) protein are nested within the bcl-3 protein. Furthermore, bcl-3 antisera immunoprecipitates an in vitro-radiolabeled 37-kD (I kappa B alpha) protein. Proteins of 56 and 38 kD can be identified in HeLa cells stimulated with PMA and immunoprecipitated with bcl-3 antisera. Comparison of tryptic peptide maps of the bcl-3 protein synthesized in vitro, and p56 and p38 from HeLa cells, shows that they are all structurally related. Removal of the amino-terminal sequences of the bcl-3 protein generates a protein that inhibits the DNA binding of the p50-p65 heterodimer but, like the 37-kD (I kappa B alpha) protein, is no longer able to inhibit the binding of the p50 and p49 homodimers with kappa B DNA. We propose that the bcl-3 and 37-kD (I kappa B alpha) proteins are related and are members of the I kappa B family.

Duke Scholars

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

December 1992

Volume

6

Issue

12A

Start / End Page

2352 / 2363

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Proto-Oncogene Proteins c-rel
  • Proto-Oncogene Proteins
  • Proto-Oncogene Mas
  • Precipitin Tests
  • NF-kappa B
  • Molecular Sequence Data
  • Mice
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kerr, L. D., Duckett, C. S., Wamsley, P., Zhang, Q., Chiao, P., Nabel, G., … Verma, I. M. (1992). The proto-oncogene bcl-3 encodes an I kappa B protein. Genes Dev, 6(12A), 2352–2363. https://doi.org/10.1101/gad.6.12a.2352
Kerr, L. D., C. S. Duckett, P. Wamsley, Q. Zhang, P. Chiao, G. Nabel, T. W. McKeithan, P. A. Baeuerle, and I. M. Verma. “The proto-oncogene bcl-3 encodes an I kappa B protein.Genes Dev 6, no. 12A (December 1992): 2352–63. https://doi.org/10.1101/gad.6.12a.2352.
Kerr LD, Duckett CS, Wamsley P, Zhang Q, Chiao P, Nabel G, et al. The proto-oncogene bcl-3 encodes an I kappa B protein. Genes Dev. 1992 Dec;6(12A):2352–63.
Kerr, L. D., et al. “The proto-oncogene bcl-3 encodes an I kappa B protein.Genes Dev, vol. 6, no. 12A, Dec. 1992, pp. 2352–63. Pubmed, doi:10.1101/gad.6.12a.2352.
Kerr LD, Duckett CS, Wamsley P, Zhang Q, Chiao P, Nabel G, McKeithan TW, Baeuerle PA, Verma IM. The proto-oncogene bcl-3 encodes an I kappa B protein. Genes Dev. 1992 Dec;6(12A):2352–2363.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

December 1992

Volume

6

Issue

12A

Start / End Page

2352 / 2363

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Proto-Oncogene Proteins c-rel
  • Proto-Oncogene Proteins
  • Proto-Oncogene Mas
  • Precipitin Tests
  • NF-kappa B
  • Molecular Sequence Data
  • Mice
  • Humans