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Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.

Publication ,  Journal Article
Anderson, AR; Segura, T
Published in: Journal of visualized experiments : JoVE
October 2022

Microgels are the building blocks of microporous annealed particle (MAP) scaffolds, which serve as a platform for both in vitro cell culture and in vivo tissue repair. In these granular scaffolds, the innate porosity generated by the void space between microgels enables cell infiltration and migration. Controlling the void fraction and particle fraction is critical for MAP scaffold design, as porosity is a bioactive cue for cells. Spherical microgels can be generated on a microfluidic device for controlled size and shape and subsequently freeze-dried using methods that prevent fracturing of the polymer network. Upon rehydration, the lyophilized microgels lead to controlled particle fractions in MAP scaffolds. The implementation of these methods for microgel lyophilization has led to reproducible studies showing the effect of particle fraction on macromolecule diffusion and cell spreading. The following protocol will cover the fabrication, lyophilization, and rehydration of microgels for controlling particle fraction in MAP scaffolds, as well as annealing the microgels through bio-orthogonal crosslinking for 3D cell culture in vitro.

Duke Scholars

Published In

Journal of visualized experiments : JoVE

DOI

EISSN

1940-087X

ISSN

1940-087X

Publication Date

October 2022

Issue

188

Related Subject Headings

  • Tissue Scaffolds
  • Porosity
  • Microgels
  • Hydrogels
  • Cell Culture Techniques, Three Dimensional
  • Cell Culture Techniques
  • 3101 Biochemistry and cell biology
  • 1702 Cognitive Sciences
  • 1701 Psychology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
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Anderson, A. R., & Segura, T. (2022). Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture. Journal of Visualized Experiments : JoVE, (188). https://doi.org/10.3791/64554
Anderson, Alexa R., and Tatiana Segura. “Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.Journal of Visualized Experiments : JoVE, no. 188 (October 2022). https://doi.org/10.3791/64554.
Anderson AR, Segura T. Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture. Journal of visualized experiments : JoVE. 2022 Oct;(188).
Anderson, Alexa R., and Tatiana Segura. “Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.Journal of Visualized Experiments : JoVE, no. 188, Oct. 2022. Epmc, doi:10.3791/64554.
Anderson AR, Segura T. Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture. Journal of visualized experiments : JoVE. 2022 Oct;(188).

Published In

Journal of visualized experiments : JoVE

DOI

EISSN

1940-087X

ISSN

1940-087X

Publication Date

October 2022

Issue

188

Related Subject Headings

  • Tissue Scaffolds
  • Porosity
  • Microgels
  • Hydrogels
  • Cell Culture Techniques, Three Dimensional
  • Cell Culture Techniques
  • 3101 Biochemistry and cell biology
  • 1702 Cognitive Sciences
  • 1701 Psychology
  • 0601 Biochemistry and Cell Biology