Skip to main content

Rapid intraoperative diagnosis of pediatric brain tumors using Raman spectroscopy: A machine learning approach

Publication ,  Journal Article
Jabarkheel, R; Ho, CS; Rodrigues, AJ; Jin, MC; Parker, JJ; Mensah-Brown, K; Yecies, D; Grant, GA
Published in: Neuro-Oncology Advances
January 1, 2022

Background: Surgical resection is a mainstay in the treatment of pediatric brain tumors to achieve tissue diagnosis and tumor debulking. While maximal safe resection of tumors is desired, it can be challenging to differentiate normal brain from neoplastic tissue using only microscopic visualization, intraoperative navigation, and tactile feedback. Here, we investigate the potential for Raman spectroscopy (RS) to accurately diagnose pediatric brain tumors intraoperatively. Methods: Using a rapid acquisition RS device, we intraoperatively imaged fresh ex vivo brain tissue samples from 29 pediatric patients at the Lucile Packard Children's Hospital between October 2018 and March 2020 in a prospective fashion. Small tissue samples measuring 2-4 mm per dimension were obtained with each individual tissue sample undergoing multiple unique Raman spectra acquisitions. All tissue samples from which Raman spectra were acquired underwent individual histopathology review. A labeled dataset of 678 unique Raman spectra gathered from 160 samples was then used to develop a machine learning model capable of (1) differentiating normal brain from tumor tissue and (2) normal brain from low-grade glioma (LGG) tissue. Results: Trained logistic regression model classifiers were developed using our labeled dataset. Model performance was evaluated using leave-one-patient-out cross-validation. The area under the curve (AUC) of the receiver-operating characteristic (ROC) curve for our tumor vs normal brain model was 0.94. The AUC of the ROC curve for LGG vs normal brain was 0.91. Conclusions: Our work suggests that RS can be used to develop a machine learning-based classifier to differentiate tumor vs non-tumor tissue during resection of pediatric brain tumors.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Neuro-Oncology Advances

DOI

EISSN

2632-2498

Publication Date

January 1, 2022

Volume

4

Issue

1
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jabarkheel, R., Ho, C. S., Rodrigues, A. J., Jin, M. C., Parker, J. J., Mensah-Brown, K., … Grant, G. A. (2022). Rapid intraoperative diagnosis of pediatric brain tumors using Raman spectroscopy: A machine learning approach. Neuro-Oncology Advances, 4(1). https://doi.org/10.1093/noajnl/vdac118
Jabarkheel, R., C. S. Ho, A. J. Rodrigues, M. C. Jin, J. J. Parker, K. Mensah-Brown, D. Yecies, and G. A. Grant. “Rapid intraoperative diagnosis of pediatric brain tumors using Raman spectroscopy: A machine learning approach.” Neuro-Oncology Advances 4, no. 1 (January 1, 2022). https://doi.org/10.1093/noajnl/vdac118.
Jabarkheel R, Ho CS, Rodrigues AJ, Jin MC, Parker JJ, Mensah-Brown K, et al. Rapid intraoperative diagnosis of pediatric brain tumors using Raman spectroscopy: A machine learning approach. Neuro-Oncology Advances. 2022 Jan 1;4(1).
Jabarkheel, R., et al. “Rapid intraoperative diagnosis of pediatric brain tumors using Raman spectroscopy: A machine learning approach.” Neuro-Oncology Advances, vol. 4, no. 1, Jan. 2022. Scopus, doi:10.1093/noajnl/vdac118.
Jabarkheel R, Ho CS, Rodrigues AJ, Jin MC, Parker JJ, Mensah-Brown K, Yecies D, Grant GA. Rapid intraoperative diagnosis of pediatric brain tumors using Raman spectroscopy: A machine learning approach. Neuro-Oncology Advances. 2022 Jan 1;4(1).

Published In

Neuro-Oncology Advances

DOI

EISSN

2632-2498

Publication Date

January 1, 2022

Volume

4

Issue

1