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Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity.

Publication ,  Journal Article
Benencia, F; Courrèges, MC; Conejo-García, JR; Mohammed-Hadley, A; Coukos, G
Published in: Cancer Biol Ther
July 2006

We tested whether tumor cells were killed by replication-incompetent recombinant herpes simplex virus (HSV) d120 lacking immediate early gene ICP4 and whether HSVd120-killed tumor cells could be used directly for tumor vaccination. Vaccine efficacy was tested in TC-1, a murine adenocarcinoma transformed with HPV16 E6 and E7, and ID8-Vegf, a murine epithelial ovarian cancer model. HSVd120 killed tumor cells by apoptosis. Tumor cells infected by HSVd120 were engulfed more avidly by immature DCs and induced DC maturation more efficiently than tumor cells killed by ultraviolet B (UVB) radiation. HSVd120 infection induced stronger upregulation of GRP94 than UVB in cells undergoing apoptosis. Immunization of mice with HSVd120-killed cells elicited stronger antitumor T cell response, including tumor reactive interferon-gamma secreting and cytotoxic T cells, and resulted in significantly stronger delay in tumor growth than immunization with UVB-killed tumor cells. Thus, the use of replication-incompetent HSV strains lacking ICP4 offers possible advantages in the preparation of whole tumor cell antigen for direct tumor vaccination.

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Published In

Cancer Biol Ther

DOI

ISSN

1538-4047

Publication Date

July 2006

Volume

5

Issue

7

Start / End Page

867 / 874

Location

United States

Related Subject Headings

  • Virus Replication
  • Vaccination
  • Up-Regulation
  • T-Lymphocytes
  • Simplexvirus
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Mice
  • Membrane Glycoproteins
  • Immediate-Early Proteins
 

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Benencia, F., Courrèges, M. C., Conejo-García, J. R., Mohammed-Hadley, A., & Coukos, G. (2006). Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity. Cancer Biol Ther, 5(7), 867–874. https://doi.org/10.4161/cbt.5.7.2908
Benencia, Fabian, Maria C. Courrèges, José R. Conejo-García, Alisha Mohammed-Hadley, and George Coukos. “Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity.Cancer Biol Ther 5, no. 7 (July 2006): 867–74. https://doi.org/10.4161/cbt.5.7.2908.
Benencia F, Courrèges MC, Conejo-García JR, Mohammed-Hadley A, Coukos G. Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity. Cancer Biol Ther. 2006 Jul;5(7):867–74.
Benencia, Fabian, et al. “Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity.Cancer Biol Ther, vol. 5, no. 7, July 2006, pp. 867–74. Pubmed, doi:10.4161/cbt.5.7.2908.
Benencia F, Courrèges MC, Conejo-García JR, Mohammed-Hadley A, Coukos G. Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity. Cancer Biol Ther. 2006 Jul;5(7):867–874.

Published In

Cancer Biol Ther

DOI

ISSN

1538-4047

Publication Date

July 2006

Volume

5

Issue

7

Start / End Page

867 / 874

Location

United States

Related Subject Headings

  • Virus Replication
  • Vaccination
  • Up-Regulation
  • T-Lymphocytes
  • Simplexvirus
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Mice
  • Membrane Glycoproteins
  • Immediate-Early Proteins