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Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome.

Publication ,  Journal Article
Anadon, CM; Zhang, C; Wang, X; Cen, L; Conejo-Garcia, JR; Yu, X
Published in: STAR Protoc
September 16, 2022

Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8+ TILs in tumors by single-cell RNA-seq paired to VDJ profiling and chromatin structure including dissociation of tumor biopsies. We have also provided guidance for subsequent fluorescence-activated cell sorting (FACS), single-cell encapsulation, bioinformatics analysis, and troubleshooting. For complete details on the use and execution of this protocol, please refer to Anadon et al. (2022).

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Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

September 16, 2022

Volume

3

Issue

3

Start / End Page

101649

Location

United States

Related Subject Headings

  • Neoplasms
  • Lymphocytes, Tumor-Infiltrating
  • Humans
  • Flow Cytometry
  • CD8-Positive T-Lymphocytes
 

Citation

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Anadon, C. M., Zhang, C., Wang, X., Cen, L., Conejo-Garcia, J. R., & Yu, X. (2022). Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome. STAR Protoc, 3(3), 101649. https://doi.org/10.1016/j.xpro.2022.101649
Anadon, Carmen M., Chaomei Zhang, Xuefeng Wang, Ling Cen, Jose R. Conejo-Garcia, and Xiaoqing Yu. “Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome.STAR Protoc 3, no. 3 (September 16, 2022): 101649. https://doi.org/10.1016/j.xpro.2022.101649.
Anadon, Carmen M., et al. “Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome.STAR Protoc, vol. 3, no. 3, Sept. 2022, p. 101649. Pubmed, doi:10.1016/j.xpro.2022.101649.
Journal cover image

Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

September 16, 2022

Volume

3

Issue

3

Start / End Page

101649

Location

United States

Related Subject Headings

  • Neoplasms
  • Lymphocytes, Tumor-Infiltrating
  • Humans
  • Flow Cytometry
  • CD8-Positive T-Lymphocytes