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The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons.

Publication ,  Journal Article
Duan, Q; Estrella, R; Carson, A; Chen, Y; Volkan, PC
Published in: G3 (Bethesda, Md.)
April 2023

Bacteriophage integrase-directed insertion of transgenic constructs into specific genomic loci has been widely used by Drosophila community. The attP40 landing site located on the second chromosome gained popularity because of its high inducible transgene expression levels. Here, unexpectedly, we found that homozygous attP40 chromosome disrupts normal glomerular organization of Or47b olfactory receptor neuron (ORN) class in Drosophila. This effect is not likely to be caused by the loss of function of Msp300, where the attP40 docking site is inserted. Moreover, the attP40 background seems to genetically interact with the second chromosome Or47b-GAL4 driver, which results in a similar glomerular defect. Whether the ORN phenotype is caused by the neighbouring genes around Msp300 locus in the presence of attP40-based insertions or a second unknown mutation in the attP40 background remains elusive. Our findings tell a cautionary tale about using this popular transgenic landing site, highlighting the importance of rigorous controls to rule out the attP40 landing site-associated background effects.

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Published In

G3 (Bethesda, Md.)

DOI

EISSN

2160-1836

ISSN

2160-1836

Publication Date

April 2023

Volume

13

Issue

4

Start / End Page

jkad022

Related Subject Headings

  • Olfactory Receptor Neurons
  • Mutation
  • Drosophila Proteins
  • Drosophila
  • Animals, Genetically Modified
  • Animals
  • 4905 Statistics
  • 3105 Genetics
  • 3101 Biochemistry and cell biology
  • 0604 Genetics
 

Citation

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Duan, Q., Estrella, R., Carson, A., Chen, Y., & Volkan, P. C. (2023). The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons. G3 (Bethesda, Md.), 13(4), jkad022. https://doi.org/10.1093/g3journal/jkad022
Duan, Qichen, Rachel Estrella, Allison Carson, Yang Chen, and Pelin C. Volkan. “The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons.G3 (Bethesda, Md.) 13, no. 4 (April 2023): jkad022. https://doi.org/10.1093/g3journal/jkad022.
Duan Q, Estrella R, Carson A, Chen Y, Volkan PC. The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons. G3 (Bethesda, Md). 2023 Apr;13(4):jkad022.
Duan, Qichen, et al. “The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons.G3 (Bethesda, Md.), vol. 13, no. 4, Apr. 2023, p. jkad022. Epmc, doi:10.1093/g3journal/jkad022.
Duan Q, Estrella R, Carson A, Chen Y, Volkan PC. The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons. G3 (Bethesda, Md). 2023 Apr;13(4):jkad022.

Published In

G3 (Bethesda, Md.)

DOI

EISSN

2160-1836

ISSN

2160-1836

Publication Date

April 2023

Volume

13

Issue

4

Start / End Page

jkad022

Related Subject Headings

  • Olfactory Receptor Neurons
  • Mutation
  • Drosophila Proteins
  • Drosophila
  • Animals, Genetically Modified
  • Animals
  • 4905 Statistics
  • 3105 Genetics
  • 3101 Biochemistry and cell biology
  • 0604 Genetics