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Membrane surfaces regulate assembly of ribonucleoprotein condensates.

Publication ,  Journal Article
Snead, WT; Jalihal, AP; Gerbich, TM; Seim, I; Hu, Z; Gladfelter, AS
Published in: Nat Cell Biol
April 2022

Biomolecular condensates organize biochemistry, yet little is known about how cells control the position and scale of these structures. In cells, condensates often appear as relatively small assemblies that do not coarsen into a single droplet despite their propensity to fuse. Here, we report that ribonucleoprotein condensates of the glutamine-rich protein Whi3 interact with the endoplasmic reticulum, which prompted us to examine how membrane association controls condensate size. Reconstitution revealed that membrane recruitment promotes Whi3 condensation under physiological conditions. These assemblies rapidly arrest, resembling size distributions seen in cells. The temporal ordering of molecular interactions and the slow diffusion of membrane-bound complexes can limit condensate size. Our experiments reveal a trade-off between locally enhanced protein concentration at membranes, which favours condensation, and an accompanying reduction in diffusion, which restricts coarsening. Given that many condensates bind endomembranes, we predict that the biophysical properties of lipid bilayers are key for controlling condensate sizes throughout the cell.

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Published In

Nat Cell Biol

DOI

EISSN

1476-4679

Publication Date

April 2022

Volume

24

Issue

4

Start / End Page

461 / 470

Location

England

Related Subject Headings

  • Ribonucleoproteins
  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

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Snead, W. T., Jalihal, A. P., Gerbich, T. M., Seim, I., Hu, Z., & Gladfelter, A. S. (2022). Membrane surfaces regulate assembly of ribonucleoprotein condensates. Nat Cell Biol, 24(4), 461–470. https://doi.org/10.1038/s41556-022-00882-3
Snead, Wilton T., Ameya P. Jalihal, Therese M. Gerbich, Ian Seim, Zhongxiu Hu, and Amy S. Gladfelter. “Membrane surfaces regulate assembly of ribonucleoprotein condensates.Nat Cell Biol 24, no. 4 (April 2022): 461–70. https://doi.org/10.1038/s41556-022-00882-3.
Snead WT, Jalihal AP, Gerbich TM, Seim I, Hu Z, Gladfelter AS. Membrane surfaces regulate assembly of ribonucleoprotein condensates. Nat Cell Biol. 2022 Apr;24(4):461–70.
Snead, Wilton T., et al. “Membrane surfaces regulate assembly of ribonucleoprotein condensates.Nat Cell Biol, vol. 24, no. 4, Apr. 2022, pp. 461–70. Pubmed, doi:10.1038/s41556-022-00882-3.
Snead WT, Jalihal AP, Gerbich TM, Seim I, Hu Z, Gladfelter AS. Membrane surfaces regulate assembly of ribonucleoprotein condensates. Nat Cell Biol. 2022 Apr;24(4):461–470.

Published In

Nat Cell Biol

DOI

EISSN

1476-4679

Publication Date

April 2022

Volume

24

Issue

4

Start / End Page

461 / 470

Location

England

Related Subject Headings

  • Ribonucleoproteins
  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences
  • 06 Biological Sciences