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Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria.

Publication ,  Journal Article
Rogers, NMK; McCumber, AW; McMillan, HM; McNamara, RP; Dittmer, DP; Kuehn, MJ; Hendren, CO; Wiesner, MR
Published in: Colloids Surf B Biointerfaces
May 2023

Extracellular vesicles (EVs) are nano-sized, biocolloidal proteoliposomes that have been shown to be produced by all cell types studied to date and are ubiquitous in the environment. Extensive literature on colloidal particles has demonstrated the implications of surface chemistry on transport behavior. Hence, one may anticipate that physicochemical properties of EVs, particularly surface charge-associated properties, may influence EV transport and specificity of interactions with surfaces. Here we compare the surface chemistry of EVs as expressed by zeta potential (calculated from electrophoretic mobility measurements). The zeta potentials of EVs produced by Pseudomonas fluorescens, Staphylococcus aureus, and Saccharomyces cerevisiae were largely unaffected by changes in ionic strength and electrolyte type, but were affected by changes in pH. The addition of humic acid altered the calculated zeta potential of the EVs, especially for those from S. cerevisiae. Differences in zeta potential were compared between EVs and their respective parent cell with no consistent trend emerging; however, significant differences were discovered between the different cell types and their EVs. These findings imply that, while EV surface charge (as estimated from zeta potential) is relatively insensitive to the evaluated environmental conditions, EVs from different organisms can differ regarding which conditions will cause colloidal instability.

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Published In

Colloids Surf B Biointerfaces

DOI

EISSN

1873-4367

Publication Date

May 2023

Volume

225

Start / End Page

113249

Location

Netherlands

Related Subject Headings

  • Saccharomyces cerevisiae
  • Extracellular Vesicles
  • Chemical Physics
  • Bacteria
  • 4004 Chemical engineering
  • 4003 Biomedical engineering
  • 3406 Physical chemistry
  • 0904 Chemical Engineering
  • 0903 Biomedical Engineering
  • 0306 Physical Chemistry (incl. Structural)
 

Citation

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Rogers, N. M. K., McCumber, A. W., McMillan, H. M., McNamara, R. P., Dittmer, D. P., Kuehn, M. J., … Wiesner, M. R. (2023). Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria. Colloids Surf B Biointerfaces, 225, 113249. https://doi.org/10.1016/j.colsurfb.2023.113249
Rogers, Nicholas M. K., Alexander W. McCumber, Hannah M. McMillan, Ryan P. McNamara, Dirk P. Dittmer, Meta J. Kuehn, Christine Ogilvie Hendren, and Mark R. Wiesner. “Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria.Colloids Surf B Biointerfaces 225 (May 2023): 113249. https://doi.org/10.1016/j.colsurfb.2023.113249.
Rogers NMK, McCumber AW, McMillan HM, McNamara RP, Dittmer DP, Kuehn MJ, et al. Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria. Colloids Surf B Biointerfaces. 2023 May;225:113249.
Rogers, Nicholas M. K., et al. “Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria.Colloids Surf B Biointerfaces, vol. 225, May 2023, p. 113249. Pubmed, doi:10.1016/j.colsurfb.2023.113249.
Rogers NMK, McCumber AW, McMillan HM, McNamara RP, Dittmer DP, Kuehn MJ, Hendren CO, Wiesner MR. Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria. Colloids Surf B Biointerfaces. 2023 May;225:113249.
Journal cover image

Published In

Colloids Surf B Biointerfaces

DOI

EISSN

1873-4367

Publication Date

May 2023

Volume

225

Start / End Page

113249

Location

Netherlands

Related Subject Headings

  • Saccharomyces cerevisiae
  • Extracellular Vesicles
  • Chemical Physics
  • Bacteria
  • 4004 Chemical engineering
  • 4003 Biomedical engineering
  • 3406 Physical chemistry
  • 0904 Chemical Engineering
  • 0903 Biomedical Engineering
  • 0306 Physical Chemistry (incl. Structural)