Skip to main content
release_alert
Welcome to the new Scholars 3.0! Read about new features and let us know what you think.
cancel
Journal cover image

Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches.

Publication ,  Journal Article
Kim, JH; Moreau, JA; Zina, JM; Mazgaeen, L; Yoon, KS; Pittendrigh, BR; Clark, JM
Published in: Pesticide Biochemistry and Physiology
October 2018

4,4'-dichlorodiphenyltrichloroethane (DDT) has been re-recommended by the World Health Organization for malaria mosquito control. Previous DDT use has resulted in resistance, and with continued use resistance will likely increase in terms of level and extent. Drosophila melanogaster is a model dipteran with a well annotated genome allowing both forward and reverse genetic manipulation, numerous studies done on insecticide resistance mechanisms, and is related to malaria mosquitoes allowing for extrapolation. The 91-R strain of D. melanogaster is highly resistant to DDT (>1500-fold) and recently, reduced penetration, increased detoxification, and direct excretion have been identified as resistance mechanisms. Their interactions, however, remain unclear. Use of Gal4/UAS-RNAi transgenic lines of D. melanogaster allowed for the targeted knockdown of genes putatively involved in DDT resistance and has identified the role of several cuticular proteins (Cyp4g1 and Lcp1), cytochrome P450 monooxygenases (Cyp6g1 and Cyp12d1), and ATP binding cassette transporters (Mdr50, Mdr65, and Mrp1) involved in decreased sensitivity to DDT. These above findings have been further validated in 91-R flies using a nanoparticle-enhanced RNAi strategy, directly implication these genes in DDT resistance in 91-R flies.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Pesticide Biochemistry and Physiology

DOI

EISSN

1095-9939

ISSN

0048-3575

Publication Date

October 2018

Volume

151

Start / End Page

90 / 99

Related Subject Headings

  • RNA Interference
  • Insecticides
  • Insecticide Resistance
  • Entomology
  • Drosophila melanogaster
  • Drosophila Proteins
  • DDT
  • Animals
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kim, J. H., Moreau, J. A., Zina, J. M., Mazgaeen, L., Yoon, K. S., Pittendrigh, B. R., & Clark, J. M. (2018). Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches. Pesticide Biochemistry and Physiology, 151, 90–99. https://doi.org/10.1016/j.pestbp.2018.03.003
Kim, Ju Hyeon, Joseph A. Moreau, Jake M. Zina, Lalita Mazgaeen, Kyong Sup Yoon, Barry R. Pittendrigh, and J Marshall Clark. “Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches.Pesticide Biochemistry and Physiology 151 (October 2018): 90–99. https://doi.org/10.1016/j.pestbp.2018.03.003.
Kim JH, Moreau JA, Zina JM, Mazgaeen L, Yoon KS, Pittendrigh BR, et al. Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches. Pesticide Biochemistry and Physiology. 2018 Oct;151:90–9.
Kim, Ju Hyeon, et al. “Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches.Pesticide Biochemistry and Physiology, vol. 151, Oct. 2018, pp. 90–99. Epmc, doi:10.1016/j.pestbp.2018.03.003.
Kim JH, Moreau JA, Zina JM, Mazgaeen L, Yoon KS, Pittendrigh BR, Clark JM. Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches. Pesticide Biochemistry and Physiology. 2018 Oct;151:90–99.
Journal cover image

Published In

Pesticide Biochemistry and Physiology

DOI

EISSN

1095-9939

ISSN

0048-3575

Publication Date

October 2018

Volume

151

Start / End Page

90 / 99

Related Subject Headings

  • RNA Interference
  • Insecticides
  • Insecticide Resistance
  • Entomology
  • Drosophila melanogaster
  • Drosophila Proteins
  • DDT
  • Animals
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences