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Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations.

Publication ,  Journal Article
Leong, KWC; Gautam, SS; Pradhan, M; Singh, YI; Kc, R; Rajbhandari, SK; Ghimire, GR; Adhikari, K; Shrestha, U; Chaudhary, R; Ghimire, G ...
Published in: Genomics
March 2022

Nepal exhibits a tuberculosis (TB) incidence rate that is comparable to neighbouring high TB incidence countries. In addition, it records >500 cases of multi-drug resistant (MDR) TB each year. The objective of this study was to perform whole-genome bioinformatic analysis on MDR-TB isolates from Nepal (n = 19) to identify the specific mutations underlying their phenotypic resistance. In addition, we examined the dominant genotype among the Nepal MDR-TB isolates, the East-Asian Beijing sub-lineage, to determine its relatedness to a panel of 1274 genomes of international strains available from public databases. These analyses provided evidence that the XDR-TB isolates in our collection were not derived from importation of primary XDR-TB to Nepal but were more likely the result of acquisition of second-line drug resistance in Nepal. Resistance to fluoroquinolones was detected among a high proportion of the Nepal isolates. This has implications for the management of TB, including appropriate antimicrobial stewardship and susceptibility testing for fluoroquinolones and other second-line TB drugs, to minimise the development of XDR-TB among Nepal TB cases.

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Published In

Genomics

DOI

EISSN

1089-8646

ISSN

0888-7543

Publication Date

March 2022

Volume

114

Issue

2

Start / End Page

110278

Related Subject Headings

  • Tuberculosis, Multidrug-Resistant
  • Nepal
  • Mycobacterium tuberculosis
  • Humans
  • Genomics
  • Genetics & Heredity
  • Fluoroquinolones
  • Extensively Drug-Resistant Tuberculosis
  • Drug Resistance, Multiple, Bacterial
  • Antitubercular Agents
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Leong, K. W. C., Gautam, S. S., Pradhan, M., Singh, Y. I., Kc, R., Rajbhandari, S. K., … O’Toole, R. F. (2022). Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations. Genomics, 114(2), 110278. https://doi.org/10.1016/j.ygeno.2022.110278
Leong, Kelvin W. C., Sanjay S. Gautam, Manoj Pradhan, Y Ibotomba Singh, Rajendra Kc, Sagar K. Rajbhandari, Gokarna R. Ghimire, et al. “Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations.Genomics 114, no. 2 (March 2022): 110278. https://doi.org/10.1016/j.ygeno.2022.110278.
Leong KWC, Gautam SS, Pradhan M, Singh YI, Kc R, Rajbhandari SK, et al. Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations. Genomics. 2022 Mar;114(2):110278.
Leong, Kelvin W. C., et al. “Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations.Genomics, vol. 114, no. 2, Mar. 2022, p. 110278. Epmc, doi:10.1016/j.ygeno.2022.110278.
Leong KWC, Gautam SS, Pradhan M, Singh YI, Kc R, Rajbhandari SK, Ghimire GR, Adhikari K, Shrestha U, Chaudhary R, Ghimire G, Khadka S, O’Toole RF. Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations. Genomics. 2022 Mar;114(2):110278.
Journal cover image

Published In

Genomics

DOI

EISSN

1089-8646

ISSN

0888-7543

Publication Date

March 2022

Volume

114

Issue

2

Start / End Page

110278

Related Subject Headings

  • Tuberculosis, Multidrug-Resistant
  • Nepal
  • Mycobacterium tuberculosis
  • Humans
  • Genomics
  • Genetics & Heredity
  • Fluoroquinolones
  • Extensively Drug-Resistant Tuberculosis
  • Drug Resistance, Multiple, Bacterial
  • Antitubercular Agents