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Histological assessment of intestinal injury by ionizing radiation.

Publication ,  Chapter
Kuo, H-C; Daniel, AR; Driver, LM; Lee, C-L; Kirsch, DG
2023

Given the potential risk of radiological terrorism and disasters, it is essential to develop plans to prepare for such events. In these hazardous scenarios, radiation-induced gastrointestinal (GI) syndrome is one of the many manifestations that may happen after the organism is exposed to a lethal dose of ionizing radiation. Therefore, it is critical to better understand how the intestinal tissues initiate and orchestrate regeneration following severe radiation injury. In this chapter, we aimed to provide several key considerations for researchers who utilize histological assessment to study radiation-induced intestinal injury. Rigor and reproducibility are critical in experimental design and can be achieved by maintaining proper radiation administration, maintaining consistency in sample collection, and selecting and using appropriate controls. We also provided technical details of histological preparation of the intestines with tips on dissecting, cleaning, fixing, and preserving. Step-by-step descriptions of both bundling and Swiss rolling are provided with discussion on how to choose between the two approaches. In the following section, we detailed several histological assessment methods and then provided suggestions on how to use histological assessment to study cellular dynamics in the small intestines. Finally, we touched on some non-histological assessments. We hope that the information provided in this chapter will contribute to the research society of radiation-induced intestinal injury with an ultimate goal of promoting the development of radiation countermeasures against the GI acute radiation syndrome.

Duke Scholars

DOI

Publication Date

2023

Volume

180

Start / End Page

147 / 175

Related Subject Headings

  • Reproducibility of Results
  • Radiation, Ionizing
  • Intestines
  • Intestine, Small
  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Kuo, H.-C., Daniel, A. R., Driver, L. M., Lee, C.-L., & Kirsch, D. G. (2023). Histological assessment of intestinal injury by ionizing radiation. (Vol. 180, pp. 147–175). https://doi.org/10.1016/bs.mcb.2023.03.001
Kuo, Hsuan-Cheng, Andrea R. Daniel, Lucy M. Driver, Chang-Lung Lee, and David G. Kirsch. “Histological assessment of intestinal injury by ionizing radiation.,” 180:147–75, 2023. https://doi.org/10.1016/bs.mcb.2023.03.001.
Kuo H-C, Daniel AR, Driver LM, Lee C-L, Kirsch DG. Histological assessment of intestinal injury by ionizing radiation. In 2023. p. 147–75.
Kuo, Hsuan-Cheng, et al. Histological assessment of intestinal injury by ionizing radiation. Vol. 180, 2023, pp. 147–75. Pubmed, doi:10.1016/bs.mcb.2023.03.001.
Kuo H-C, Daniel AR, Driver LM, Lee C-L, Kirsch DG. Histological assessment of intestinal injury by ionizing radiation. 2023. p. 147–175.

DOI

Publication Date

2023

Volume

180

Start / End Page

147 / 175

Related Subject Headings

  • Reproducibility of Results
  • Radiation, Ionizing
  • Intestines
  • Intestine, Small
  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology