Skip to main content

Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology.

Publication ,  Journal Article
Langenbacher, AD; Lu, F; Crisman, L; Huang, ZYS; Chapski, DJ; Vondriska, TM; Wang, Y; Gao, C; Chen, J-N
Published in: J Cardiovasc Dev Dis
May 20, 2023

The PAF1 complex component Rtf1 is an RNA Polymerase II-interacting transcription regulatory protein that promotes transcription elongation and the co-transcriptional monoubiquitination of histone 2B. Rtf1 plays an essential role in the specification of cardiac progenitors from the lateral plate mesoderm during early embryogenesis, but its requirement in mature cardiac cells is unknown. Here, we investigate the importance of Rtf1 in neonatal and adult cardiomyocytes using knockdown and knockout approaches. We demonstrate that loss of Rtf1 activity in neonatal cardiomyocytes disrupts cell morphology and results in a breakdown of sarcomeres. Similarly, Rtf1 ablation in mature cardiomyocytes of the adult mouse heart leads to myofibril disorganization, disrupted cell-cell junctions, fibrosis, and systolic dysfunction. Rtf1 knockout hearts eventually fail and exhibit structural and gene expression defects resembling dilated cardiomyopathy. Intriguingly, we observed that loss of Rtf1 activity causes a rapid change in the expression of key cardiac structural and functional genes in both neonatal and adult cardiomyocytes, suggesting that Rtf1 is continuously required to support expression of the cardiac gene program.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Cardiovasc Dev Dis

DOI

EISSN

2308-3425

Publication Date

May 20, 2023

Volume

10

Issue

5

Location

Switzerland

Related Subject Headings

  • 3201 Cardiovascular medicine and haematology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Langenbacher, A. D., Lu, F., Crisman, L., Huang, Z. Y. S., Chapski, D. J., Vondriska, T. M., … Chen, J.-N. (2023). Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology. J Cardiovasc Dev Dis, 10(5). https://doi.org/10.3390/jcdd10050221
Langenbacher, Adam D., Fei Lu, Lauren Crisman, Zi Yi Stephanie Huang, Douglas J. Chapski, Thomas M. Vondriska, Yibin Wang, Chen Gao, and Jau-Nian Chen. “Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology.J Cardiovasc Dev Dis 10, no. 5 (May 20, 2023). https://doi.org/10.3390/jcdd10050221.
Langenbacher AD, Lu F, Crisman L, Huang ZYS, Chapski DJ, Vondriska TM, et al. Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology. J Cardiovasc Dev Dis. 2023 May 20;10(5).
Langenbacher, Adam D., et al. “Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology.J Cardiovasc Dev Dis, vol. 10, no. 5, May 2023. Pubmed, doi:10.3390/jcdd10050221.
Langenbacher AD, Lu F, Crisman L, Huang ZYS, Chapski DJ, Vondriska TM, Wang Y, Gao C, Chen J-N. Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology. J Cardiovasc Dev Dis. 2023 May 20;10(5).

Published In

J Cardiovasc Dev Dis

DOI

EISSN

2308-3425

Publication Date

May 20, 2023

Volume

10

Issue

5

Location

Switzerland

Related Subject Headings

  • 3201 Cardiovascular medicine and haematology