Supplementary Figure S2 from EGFR Inhibition by Cetuximab Modulates Hypoxia and IFN Response Genes in Head and Neck Squamous Cell Carcinoma

<p>(A, B) Representative mIHC images showing staining of nuclei (blue), tumor cell (orange - PCK) and selected immune checkpoint markers in each molecular subgroup. (A) Images show four lymphoid markers - CD3+ T-cells (Green), CD8+ cytotoxic T-cells (Yellow), CD69+ activated T-cells (Red) and CD103+ resident memory T-cells (Cyan). (B) Images show three myeloid markers – HLA-DR+ APCs (Green), MHCII+ APCs (Cyan) and CD163+ M2 macrophages (Yellow). (C) Linear model showing correlation between PD-L1 CPS and Immune, Mixture and Hypoxia subgroups. (D-E) Boxplots showing cell counts distribution of different immune markers among the primary (n = 63) and recurrent (n = 20) tumors in TMA mIHC cohort. Students t-tests were used to test for significant differences between the groups.</p>

Duke Scholars

Author Jose Ramon Conejo-Garcia Integrative Immunobiology