Scholars@Duke
Journal cover image

Regulation of homologous recombination by RNF20-dependent H2B ubiquitination.

Publication ,  Journal Article
Nakamura, K; Kato, A; Kobayashi, J; Yanagihara, H; Sakamoto, S; Oliveira, DVNP; Shimada, M; Tauchi, H; Suzuki, H; Tashiro, S; Zou, L; Komatsu, K
Published in: Mol Cell
March 4, 2011
Published version (DOI) Link to item

The E3 ubiquitin ligase RNF20 regulates chromatin structure by monoubiquitinating histone H2B in transcription. Here, we show that RNF20 is localized to double-stranded DNA breaks (DSBs) independently of H2AX and is required for the DSB-induced H2B ubiquitination. In addition, RNF20 is required for the methylation of H3K4 at DSBs and the recruitment of the chromatin-remodeling factor SNF2h. Depletion of RNF20, depletion of SNF2h, or expression of the H2B mutant lacking the ubiquitination site (K120R) compromises resection of DNA ends and recruitment of RAD51 and BRCA1. Consequently, cells lacking RNF20 or SNF2h and cells expressing H2B K120R exhibit pronounced defects in homologous recombination repair (HRR) and enhanced sensitivity to radiation. Finally, the function of RNF20 in HRR can be partially bypassed by forced chromatin relaxation. Thus, the RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling.

Duke Scholars

Lee Zou
Author Lee Zou Pharmacology & Cancer Biology
Altmetric Attention Stats
Dimensions Citation Stats

Published In

Mol Cell

DOI

10.1016/j.molcel.2011.02.002

EISSN

1097-4164

Publication Date

March 4, 2011

Volume

41

Issue

5

Start / End Page

515 / 528

Location

United States

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Ubiquitin
  • Transcription, Genetic
  • Time Factors
  • Recombination, Genetic
  • Rad51 Recombinase
  • Nijmegen Breakage Syndrome
  • Humans
  • Histones
  • Hela Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nakamura, K., Kato, A., Kobayashi, J., Yanagihara, H., Sakamoto, S., Oliveira, D. V. N. P., … Komatsu, K. (2011). Regulation of homologous recombination by RNF20-dependent H2B ubiquitination. Mol Cell, 41(5), 515–528. https://doi.org/10.1016/j.molcel.2011.02.002
Nakamura, Kyosuke, Akihiro Kato, Junya Kobayashi, Hiromi Yanagihara, Shuichi Sakamoto, Douglas V. N. P. Oliveira, Mikio Shimada, et al. “Regulation of homologous recombination by RNF20-dependent H2B ubiquitination.” Mol Cell 41, no. 5 (March 4, 2011): 515–28. https://doi.org/10.1016/j.molcel.2011.02.002.
Nakamura K, Kato A, Kobayashi J, Yanagihara H, Sakamoto S, Oliveira DVNP, et al. Regulation of homologous recombination by RNF20-dependent H2B ubiquitination. Mol Cell. 2011 Mar 4;41(5):515–28.
Nakamura, Kyosuke, et al. “Regulation of homologous recombination by RNF20-dependent H2B ubiquitination.” Mol Cell, vol. 41, no. 5, Mar. 2011, pp. 515–28. Pubmed, doi:10.1016/j.molcel.2011.02.002.
Nakamura K, Kato A, Kobayashi J, Yanagihara H, Sakamoto S, Oliveira DVNP, Shimada M, Tauchi H, Suzuki H, Tashiro S, Zou L, Komatsu K. Regulation of homologous recombination by RNF20-dependent H2B ubiquitination. Mol Cell. 2011 Mar 4;41(5):515–528.
Journal cover image

Published In

Mol Cell

DOI

10.1016/j.molcel.2011.02.002

EISSN

1097-4164

Publication Date

March 4, 2011

Volume

41

Issue

5

Start / End Page

515 / 528

Location

United States

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Ubiquitin
  • Transcription, Genetic
  • Time Factors
  • Recombination, Genetic
  • Rad51 Recombinase
  • Nijmegen Breakage Syndrome
  • Humans
  • Histones
  • Hela Cells