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Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues

Publication ,  Journal Article
Park, K; Jayadev, R; Payne, SG; Kenny-Ganzert, IW; Chi, Q; Costa, DS; Ramos-Lewis, W; Thendral, SB; Sherwood, DR
Published in: eLife
July 5, 2023

Separate tissues connect through adjoining basement membranes to carry out molecular barrier, exchange, and organ support functions. Cell adhesion at these connections must be robust and balanced to withstand independent tissue movement. Yet, how cells achieve synchronized adhesion to connect tissues is unknown. Here, we have investigated this question using the utse-seam tissue connection that supports the uterus during egg-laying. Through genetics, quantitative fluorescence, and cell-specific molecular disruption, we show that type IV collagen, which fastens the linkage, also activates the collagen receptor discoidin domain receptor-2 (DDR-2) in both the utse and seam. RNAi depletion, genome editing, and photobleaching experiments revealed that DDR-2 signals through LET-60/Ras to coordinately strengthen an integrin adhesion in the utse and seam that stabilizes their connection. These results uncover a synchronizing mechanism for robust adhesion during tissue connection, where collagen both affixes the linkage and signals to both tissues to bolster their adhesion.

Duke Scholars

Published In

eLife

DOI

EISSN

2050-084X

Publication Date

July 5, 2023

Volume

12

Publisher

eLife Sciences Publications, Ltd

Related Subject Headings

  • 0601 Biochemistry and Cell Biology
 

Citation

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Park, K., Jayadev, R., Payne, S. G., Kenny-Ganzert, I. W., Chi, Q., Costa, D. S., … Sherwood, D. R. (2023). Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues. ELife, 12. https://doi.org/10.7554/elife.87037.3
Park, Kieop, Ranjay Jayadev, Sara G. Payne, Isabel W. Kenny-Ganzert, Qiuyi Chi, Daniel S. Costa, William Ramos-Lewis, Siddharthan B. Thendral, and David R. Sherwood. “Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues.” ELife 12 (July 5, 2023). https://doi.org/10.7554/elife.87037.3.
Park K, Jayadev R, Payne SG, Kenny-Ganzert IW, Chi Q, Costa DS, et al. Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues. eLife. 2023 Jul 5;12.
Park, Kieop, et al. “Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues.” ELife, vol. 12, eLife Sciences Publications, Ltd, July 2023. Crossref, doi:10.7554/elife.87037.3.
Park K, Jayadev R, Payne SG, Kenny-Ganzert IW, Chi Q, Costa DS, Ramos-Lewis W, Thendral SB, Sherwood DR. Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues. eLife. eLife Sciences Publications, Ltd; 2023 Jul 5;12.

Published In

eLife

DOI

EISSN

2050-084X

Publication Date

July 5, 2023

Volume

12

Publisher

eLife Sciences Publications, Ltd

Related Subject Headings

  • 0601 Biochemistry and Cell Biology