Collagen fiber structure guides 3D motility of cytotoxic T lymphocytes.
Lymphocyte motility is governed by a complex array of mechanisms, and highly dependent on external microenvironmental cues. Tertiary lymphoid sites in particular have unique physical structure such as collagen fiber alignment, due to matrix deposition and remodeling. Three dimensional studies of human lymphocytes in such environments are lacking. We hypothesized that aligned collagenous environment modulates CD8+ T cells motility. We encapsulated activated CD8+ T cells in collagen hydrogels of distinct fiber alignment, a characteristic of tumor microenvironments. We found that human CD8+ T cells move faster and more persistently in aligned collagen fibers compared with nonaligned collagen fibers. Moreover, CD8+ T cells move along the axis of collagen alignment. We showed that myosin light chain kinase (MLCK) inhibition could nullify the effect of aligned collagen on CD8+ T cell motility patterns by decreasing T cell turning in unaligned collagen fiber gels. Finally, as an example of a tertiary lymphoid site, we found that xenograft prostate tumors exhibit highly aligned collagen fibers. We observed CD8+ T cells alongside aligned collagen fibers, and found that they are mostly concentrated in the periphery of tumors. Overall, using an in vitro controlled hydrogel system, we show that collagen fiber organization modulates CD8+ T cells movement via MLCK activation thus providing basis for future studies into relevant therapeutics.
Duke Scholars
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Related Subject Headings
- T-Lymphocytes, Cytotoxic
- Prostatic Neoplasms
- Neoplasm Transplantation
- Myosin-Light-Chain Kinase
- Mice
- Male
- Lab-On-A-Chip Devices
- Humans
- Extracellular Matrix
- Collagen
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Start / End Page
Related Subject Headings
- T-Lymphocytes, Cytotoxic
- Prostatic Neoplasms
- Neoplasm Transplantation
- Myosin-Light-Chain Kinase
- Mice
- Male
- Lab-On-A-Chip Devices
- Humans
- Extracellular Matrix
- Collagen