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Urinary Amino-PAHs in relation to diesel engine emissions and urinary mutagenicity.

Publication ,  Journal Article
Zhang, JJ; Zheng, Y; Vermeulen, R; Liu, XL; Dai, Y; Hu, W; He, L; Lin, Y; Ren, D; Duan, H; Niu, Y; Xu, J; Fu, W; Meliefste, K; Zhou, B ...
Published in: International journal of hygiene and environmental health
August 2023

Diesel exhaust has long been of health concern due to established toxicity including carcinogenicity in humans. However, the precise components of diesel engine emissions that drive carcinogenesis are still unclear. Limited work has suggested that nitrated polycyclic aromatic hydrocarbons (NPAHs) such as 1-nitropyrene and 2-nitrofluorene may be more abundant in diesel exhaust. The present study aimed to examine whether urinary amino metabolites of these NPAHs were associated with high levels of diesel engine emissions and urinary mutagenicity in a group of highly exposed workers including both smokers and nonsmokers. Spot urine samples were collected immediately following a standard work shift from each of the 54 diesel engine testers and 55 non-tester controls for the analysis of five amino metabolites of NPAHs, and cotinine (a biomarker of tobacco smoke exposure) using liquid chromatography-mass spectrometry. An overnight urine sample was collected in a subgroup of non-smoking participants for mutagenicity analysis using strain YG1041 in the Salmonella (Ames) mutagenicity assay. Personal exposure to fine particles (PM2.5) and more-diesel-specific constituents (elemental carbon and soot) was assessed for the engine testers by measuring breathing-zone concentrations repeatedly over several full work shifts. Results showed that it was 12.8 times more likely to detect 1-aminopyrene and 2.9 times more likely to detect 2-aminofluorene in the engine testers than in unexposed controls. Urinary concentrations of 1-aminopyrene were significantly higher in engine testers (p < 0.001), and strongly correlated with soot and elemental carbon exposure as well as mutagenicity tested in strain YG1041 with metabolic activation (p < 0.001). Smoking did not affect 1-aminopyrene concentrations and 1-aminopyrene relationships with diesel exposure. In contrast, both engine emissions and smoking affected 2-aminofluorene concentrations. The results confirm that urinary 1-aminopyrene may serve as an exposure biomarker for diesel engine emissions and associated mutagenicity.

Duke Scholars

Published In

International journal of hygiene and environmental health

DOI

EISSN

1618-131X

ISSN

1438-4639

Publication Date

August 2023

Volume

253

Start / End Page

114223

Related Subject Headings

  • Vehicle Emissions
  • Toxicology
  • Soot
  • Polycyclic Aromatic Hydrocarbons
  • Nitrates
  • Mutagens
  • Humans
  • Epidemiology
  • Biomarkers
  • 4206 Public health
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, J. J., Zheng, Y., Vermeulen, R., Liu, X. L., Dai, Y., Hu, W., … Rothman, N. (2023). Urinary Amino-PAHs in relation to diesel engine emissions and urinary mutagenicity. International Journal of Hygiene and Environmental Health, 253, 114223. https://doi.org/10.1016/j.ijheh.2023.114223
Zhang, Junfeng Jim, Yuxin Zheng, Roel Vermeulen, Xing Lucy Liu, Yufei Dai, Wei Hu, Linchen He, et al. “Urinary Amino-PAHs in relation to diesel engine emissions and urinary mutagenicity.International Journal of Hygiene and Environmental Health 253 (August 2023): 114223. https://doi.org/10.1016/j.ijheh.2023.114223.
Zhang JJ, Zheng Y, Vermeulen R, Liu XL, Dai Y, Hu W, et al. Urinary Amino-PAHs in relation to diesel engine emissions and urinary mutagenicity. International journal of hygiene and environmental health. 2023 Aug;253:114223.
Zhang, Junfeng Jim, et al. “Urinary Amino-PAHs in relation to diesel engine emissions and urinary mutagenicity.International Journal of Hygiene and Environmental Health, vol. 253, Aug. 2023, p. 114223. Epmc, doi:10.1016/j.ijheh.2023.114223.
Zhang JJ, Zheng Y, Vermeulen R, Liu XL, Dai Y, Hu W, He L, Lin Y, Ren D, Duan H, Niu Y, Xu J, Fu W, Meliefste K, Zhou B, Yang J, Ye M, Jia X, Meng T, Bin P, Bassig BA, Hosgood HD, Silverman D, Lan Q, Rothman N. Urinary Amino-PAHs in relation to diesel engine emissions and urinary mutagenicity. International journal of hygiene and environmental health. 2023 Aug;253:114223.
Journal cover image

Published In

International journal of hygiene and environmental health

DOI

EISSN

1618-131X

ISSN

1438-4639

Publication Date

August 2023

Volume

253

Start / End Page

114223

Related Subject Headings

  • Vehicle Emissions
  • Toxicology
  • Soot
  • Polycyclic Aromatic Hydrocarbons
  • Nitrates
  • Mutagens
  • Humans
  • Epidemiology
  • Biomarkers
  • 4206 Public health