Skip to main content
Journal cover image

Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model.

Publication ,  Journal Article
Roy, R; Brodarac, A; Kukucka, M; Kurtz, A; Becher, PM; Jülke, K; Choi, Y-H; Pinzur, L; Chajut, A; Tschöpe, C; Stamm, C
Published in: J Surg Res
November 2013

BACKGROUND: Autologous cells for cell therapy of ischemic cardiomyopathy often display age- and disease-related functional impairment, whereas an allogenic immunotolerant cell product would allow off-the-shelf application of uncompromised donor cells. We investigated the cardiac regeneration potential of a novel, clinical-grade placenta-derived human stromal cell product (PLX-PAD). METHODS: PLX-PAD cells derived from human donor placentas and expanded in a three-dimensional bioreactor system were tested for surface marker expression, proangiogenic, anti-inflammatory, and immunomodulatory properties in vitro. In BALB/C mice, the left anterior descending artery was ligated and PLX-PAD cells (n = 10) or vehicle (n = 10) were injected in the infarct border zone. Four weeks later, heart function was analyzed by two-dimensional and M-mode echocardiography. Scar size, microvessel density, extracellular matrix composition, myocyte apoptosis, and PLX-PAD cell retention were studied by histology. RESULTS: In vitro, PLX-PAD cells displayed both proangiogenesis and anti-inflammatory properties, represented by the secretion of both vascular endothelial growth factor and angiopoietin-1 that was upregulated by hypoxia, as well as by the capacity to suppress T-cell proliferation and augment IL-10 secretion when co-cultured with peripheral blood mononuclear cells. Compared with control mice, PLX-PAD-treated hearts had better contractile function, smaller infarct size, greater regional left ventricular wall thickness, and less apoptosis after 4 wk. PLX-PAD stimulated both angiogenesis and arteriogenesis in the infarct border zone, and periostin expression was upregulated in PLX-PAD-treated hearts. CONCLUSIONS: Clinical-grade PLX-PAD cells exert beneficial effects on ischemic myocardium that are associated with improved contractile function, and may be suitable for further evaluation aiming at clinical pilot trials of cardiac cell therapy.

Duke Scholars

Published In

J Surg Res

DOI

EISSN

1095-8673

Publication Date

November 2013

Volume

185

Issue

1

Start / End Page

70 / 83

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Surgery
  • Stromal Cells
  • Pregnancy
  • Placenta
  • Paracrine Communication
  • Neovascularization, Physiologic
  • Myocardial Infarction
  • Mice, Inbred BALB C
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Roy, R., Brodarac, A., Kukucka, M., Kurtz, A., Becher, P. M., Jülke, K., … Stamm, C. (2013). Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model. J Surg Res, 185(1), 70–83. https://doi.org/10.1016/j.jss.2013.05.084
Roy, Rajika, Andreja Brodarac, Marian Kukucka, Andreas Kurtz, Peter Moritz Becher, Kerstin Jülke, Yeong-Hoon Choi, et al. “Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model.J Surg Res 185, no. 1 (November 2013): 70–83. https://doi.org/10.1016/j.jss.2013.05.084.
Roy R, Brodarac A, Kukucka M, Kurtz A, Becher PM, Jülke K, et al. Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model. J Surg Res. 2013 Nov;185(1):70–83.
Roy, Rajika, et al. “Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model.J Surg Res, vol. 185, no. 1, Nov. 2013, pp. 70–83. Pubmed, doi:10.1016/j.jss.2013.05.084.
Roy R, Brodarac A, Kukucka M, Kurtz A, Becher PM, Jülke K, Choi Y-H, Pinzur L, Chajut A, Tschöpe C, Stamm C. Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model. J Surg Res. 2013 Nov;185(1):70–83.
Journal cover image

Published In

J Surg Res

DOI

EISSN

1095-8673

Publication Date

November 2013

Volume

185

Issue

1

Start / End Page

70 / 83

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Surgery
  • Stromal Cells
  • Pregnancy
  • Placenta
  • Paracrine Communication
  • Neovascularization, Physiologic
  • Myocardial Infarction
  • Mice, Inbred BALB C
  • Male