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Identification of Immune Cells in Murine Olfactory Mucosa.

Publication ,  Journal Article
Wellford, SA; Moseman, EA
Published in: Methods Mol Biol
2023

Olfactory immunology is an emerging field in the context of infectious disease and neuroimmunology, yet characterization of immune cells within the murine olfactory mucosa remains sparse. This is partially due to the difficulty in distinguishing olfactory-resident immune cells from immune cells that reside within nasal turbinate bone marrow. Using techniques like intranasal antibody labeling, we have developed methods to definitively identify olfactory immune cells via flow cytometry and immunofluorescent confocal microscopy. This protocol will describe the best practices for these methods, as well as detail how intravenous antibody labeling can be used to study the blood-olfactory barrier, an important determinant of olfactory immunity. We also include validated markers for the identification of major olfactory immune populations.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2023

Volume

2710

Start / End Page

61 / 69

Location

United States

Related Subject Headings

  • Microscopy, Confocal
  • Mice
  • Immunoglobulins, Intravenous
  • Flow Cytometry
  • Developmental Biology
  • Coloring Agents
  • Antibodies
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
 

Citation

APA
Chicago
ICMJE
MLA
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Wellford, S. A., & Moseman, E. A. (2023). Identification of Immune Cells in Murine Olfactory Mucosa. Methods Mol Biol, 2710, 61–69. https://doi.org/10.1007/978-1-0716-3425-7_5
Wellford, Sebastian A., and E Ashley Moseman. “Identification of Immune Cells in Murine Olfactory Mucosa.Methods Mol Biol 2710 (2023): 61–69. https://doi.org/10.1007/978-1-0716-3425-7_5.
Wellford SA, Moseman EA. Identification of Immune Cells in Murine Olfactory Mucosa. Methods Mol Biol. 2023;2710:61–9.
Wellford, Sebastian A., and E. Ashley Moseman. “Identification of Immune Cells in Murine Olfactory Mucosa.Methods Mol Biol, vol. 2710, 2023, pp. 61–69. Pubmed, doi:10.1007/978-1-0716-3425-7_5.
Wellford SA, Moseman EA. Identification of Immune Cells in Murine Olfactory Mucosa. Methods Mol Biol. 2023;2710:61–69.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2023

Volume

2710

Start / End Page

61 / 69

Location

United States

Related Subject Headings

  • Microscopy, Confocal
  • Mice
  • Immunoglobulins, Intravenous
  • Flow Cytometry
  • Developmental Biology
  • Coloring Agents
  • Antibodies
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology