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Triphenyl phosphate-induced pericardial edema in zebrafish embryos is reversible following depuration in clean water.

Publication ,  Journal Article
Wiegand, J; Hoang, J; Avila-Barnard, S; Nemarugommula, C; Ha, M; Zhang, S; Stapleton, HM; Volz, DC
Published in: Aquatic toxicology (Amsterdam, Netherlands)
October 2023

Triphenyl phosphate (TPHP) - a widely used organophosphate-based flame retardant - blocks cardiac looping during zebrafish development in a concentration-dependent manner, a phenotype that is dependent on disruption of embryonic osmoregulation and pericardial edema formation. However, it's currently unclear whether (1) TPHP-induced effects on osmoregulation are driven by direct TPHP-induced injury to the embryonic epidermis and (2) whether TPHP-induced pericardial edema is reversible or irreversible following cessation of exposure. Therefore, the objectives of this study were to determine whether TPHP-induced pericardial edema is reversible and whether TPHP causes injury to the embryonic epidermis by quantifying the number of DAPI-positive epidermal cells and analyzing the morphology of the yolk sac epithelium using scanning electron microscopy. First, we found that exposure to 5 μM TPHP from 24-72 h post-fertilization (hpf) did not increase prolactin - a hormone that regulates ions and water levels - in embryonic zebrafish, whereas high ionic strength exposure media was associated with elevated levels of prolactin. Second, we found that exposure to 5 μM TPHP from 24-72 hpf did not decrease DAPI-positive epidermal cells within the embryonic epithelium, and that co-exposure with 2.14 μM fenretinide - a synthetic retinoid that promotes epithelial wound repair - from 24-72 hpf did not mitigate the prevalence of TPHP-induced epidermal folds within the yolk sac epithelium when embryos were exposed within high ionic strength exposure media. Finally, we found that the pericardial area and body length of embryos exposed to 5 μM TPHP from 24-72 hpf were similar to vehicle-treated embryos at 120 hpf following transfer to clean water and depuration of TPHP from 72-120 hpf. Overall, our findings suggest that (1) the ionic strength of exposure media may influence the baseline physiology of zebrafish embryos; (2) TPHP does not cause direct injury to the embryonic epidermis; and (3) TPHP-induced effects on pericardial area and body length are reversible 48 h after transferring embryos to clean water.

Duke Scholars

Published In

Aquatic toxicology (Amsterdam, Netherlands)

DOI

EISSN

1879-1514

ISSN

0166-445X

Publication Date

October 2023

Volume

263

Start / End Page

106699

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Toxicology
  • Prolactin
  • Organophosphates
  • Embryo, Nonmammalian
  • Edema
  • Animals
  • 41 Environmental sciences
  • 34 Chemical sciences
 

Citation

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MLA
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Wiegand, J., Hoang, J., Avila-Barnard, S., Nemarugommula, C., Ha, M., Zhang, S., … Volz, D. C. (2023). Triphenyl phosphate-induced pericardial edema in zebrafish embryos is reversible following depuration in clean water. Aquatic Toxicology (Amsterdam, Netherlands), 263, 106699. https://doi.org/10.1016/j.aquatox.2023.106699
Wiegand, Jenna, John Hoang, Sarah Avila-Barnard, Charvita Nemarugommula, Megan Ha, Sharon Zhang, Heather M. Stapleton, and David C. Volz. “Triphenyl phosphate-induced pericardial edema in zebrafish embryos is reversible following depuration in clean water.Aquatic Toxicology (Amsterdam, Netherlands) 263 (October 2023): 106699. https://doi.org/10.1016/j.aquatox.2023.106699.
Wiegand J, Hoang J, Avila-Barnard S, Nemarugommula C, Ha M, Zhang S, et al. Triphenyl phosphate-induced pericardial edema in zebrafish embryos is reversible following depuration in clean water. Aquatic toxicology (Amsterdam, Netherlands). 2023 Oct;263:106699.
Wiegand, Jenna, et al. “Triphenyl phosphate-induced pericardial edema in zebrafish embryos is reversible following depuration in clean water.Aquatic Toxicology (Amsterdam, Netherlands), vol. 263, Oct. 2023, p. 106699. Epmc, doi:10.1016/j.aquatox.2023.106699.
Wiegand J, Hoang J, Avila-Barnard S, Nemarugommula C, Ha M, Zhang S, Stapleton HM, Volz DC. Triphenyl phosphate-induced pericardial edema in zebrafish embryos is reversible following depuration in clean water. Aquatic toxicology (Amsterdam, Netherlands). 2023 Oct;263:106699.
Journal cover image

Published In

Aquatic toxicology (Amsterdam, Netherlands)

DOI

EISSN

1879-1514

ISSN

0166-445X

Publication Date

October 2023

Volume

263

Start / End Page

106699

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Toxicology
  • Prolactin
  • Organophosphates
  • Embryo, Nonmammalian
  • Edema
  • Animals
  • 41 Environmental sciences
  • 34 Chemical sciences