Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence.
Publication
, Journal Article
Nagai, MH; Matsunami, H
Published in: Methods Mol Biol
2023
This microscope-based method allows demonstrating that an odorant receptor responded to an odorant in vivo. In sections of olfactory epithelium from odorant-exposed mice, the subpopulation of olfactory sensory neurons expressing a particular odorant receptor type is labeled using RNA fluorescence in situ hybridization. Sequential immunofluorescence against the phosphorylated S6 ribosomal subunit reveals the activated olfactory sensory neurons. The presence of double-labeled cells confirms that the particular odorant receptor type was activated by the odorant stimulation.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2023
Volume
2710
Start / End Page
83 / 97
Location
United States
Related Subject Headings
- Receptors, Odorant
- RNA
- Olfactory Receptor Neurons
- Mice
- In Situ Hybridization, Fluorescence
- Fluorescent Antibody Technique
- Developmental Biology
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Nagai, M. H., & Matsunami, H. (2023). Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence. Methods Mol Biol, 2710, 83–97. https://doi.org/10.1007/978-1-0716-3425-7_7
Nagai, Maira Harume, and Hiroaki Matsunami. “Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence.” Methods Mol Biol 2710 (2023): 83–97. https://doi.org/10.1007/978-1-0716-3425-7_7.
Nagai MH, Matsunami H. Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence. Methods Mol Biol. 2023;2710:83–97.
Nagai, Maira Harume, and Hiroaki Matsunami. “Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence.” Methods Mol Biol, vol. 2710, 2023, pp. 83–97. Pubmed, doi:10.1007/978-1-0716-3425-7_7.
Nagai MH, Matsunami H. Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence. Methods Mol Biol. 2023;2710:83–97.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2023
Volume
2710
Start / End Page
83 / 97
Location
United States
Related Subject Headings
- Receptors, Odorant
- RNA
- Olfactory Receptor Neurons
- Mice
- In Situ Hybridization, Fluorescence
- Fluorescent Antibody Technique
- Developmental Biology
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology