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Senescent Cells with Augmented Cytokine Production for Microvascular Bioengineering and Tissue Repairs.

Publication ,  Journal Article
Xiao, Y; Liu, C; Chen, Z; Blatchley, MR; Kim, D; Zhou, J; Xu, M; Gerecht, S; Fan, R
Published in: Advanced biosystems
August 2019

Controlled delivery of cytokines and growth factors has been an area of intense research interest for molecular and cellular bioengineering, immunotherapy, and regenerative medicine. In this study, we show that primary human lung fibroblasts chemically induced to senescence (cell cycle arrest) can act as a living source to transiently produce factors essential for promoting vasculogenesis or angiogenesis, such as VEGF, HGF, and IL-8. Co-culture of senescent fibroblasts with HUVECs in a fibrin gel demonstrated accelerated formation and maturation of microvessel networks in as early as three days. Unlike the usage of non-senescent fibroblasts as the angiogenesis-promoting cells, this approach eliminates drawbacks related to the overproliferation of fibroblasts and the subsequent disruption of tissue architecture, integrity, or function. Co-culture of pancreatic islets with senescent fibroblasts and endothelial cells in a gel matrix maintains the viability and function of islets ex vivo for up to five days. Applying senescent fibroblasts to wound repair in vivo led to increased blood flow in a diabetic mouse model. Together, this work points to a new direction for engineering the delivery of cytokines and growth factors that promote microvascular tissue engineering and tissue repairs.

Duke Scholars

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Published In

Advanced biosystems

DOI

EISSN

2366-7478

ISSN

2366-7478

Publication Date

August 2019

Volume

3

Issue

8

Start / End Page

1900089
 

Citation

APA
Chicago
ICMJE
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Xiao, Y., Liu, C., Chen, Z., Blatchley, M. R., Kim, D., Zhou, J., … Fan, R. (2019). Senescent Cells with Augmented Cytokine Production for Microvascular Bioengineering and Tissue Repairs. Advanced Biosystems, 3(8), 1900089. https://doi.org/10.1002/adbi.201900089
Xiao, Yang, Chang Liu, Zhuo Chen, Michael R. Blatchley, Dongjoo Kim, Jing Zhou, Ming Xu, Sharon Gerecht, and Rong Fan. “Senescent Cells with Augmented Cytokine Production for Microvascular Bioengineering and Tissue Repairs.Advanced Biosystems 3, no. 8 (August 2019): 1900089. https://doi.org/10.1002/adbi.201900089.
Xiao Y, Liu C, Chen Z, Blatchley MR, Kim D, Zhou J, et al. Senescent Cells with Augmented Cytokine Production for Microvascular Bioengineering and Tissue Repairs. Advanced biosystems. 2019 Aug;3(8):1900089.
Xiao, Yang, et al. “Senescent Cells with Augmented Cytokine Production for Microvascular Bioengineering and Tissue Repairs.Advanced Biosystems, vol. 3, no. 8, Aug. 2019, p. 1900089. Epmc, doi:10.1002/adbi.201900089.
Xiao Y, Liu C, Chen Z, Blatchley MR, Kim D, Zhou J, Xu M, Gerecht S, Fan R. Senescent Cells with Augmented Cytokine Production for Microvascular Bioengineering and Tissue Repairs. Advanced biosystems. 2019 Aug;3(8):1900089.
Journal cover image

Published In

Advanced biosystems

DOI

EISSN

2366-7478

ISSN

2366-7478

Publication Date

August 2019

Volume

3

Issue

8

Start / End Page

1900089