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Endothelial progenitor cell recruitment in a microfluidic vascular model.

Publication ,  Journal Article
Lewis, DM; Abaci, HE; Xu, Y; Gerecht, S
Published in: Biofabrication
December 2015

During vessel injury, endothelial progenitors cells (EPCs) are recruited from bone marrow and directed to the hypoxic injury site. The hypoxic conditions in the damaged blood vessel promote TNF-α, which upregulates intercellular adhesion molecule-1 (ICAM-1). EPCs attach to endothelial cell lining using ICAM-1. Here we aimed to examine EPC attachment to ECs in an injured-blood vessel conditions. We first determined ICAM-1 expression in stimulated HUVECs. We stimulated HUVECs with 21% oxygen (atmospheric), atmospheric with TNF-α-supplemented media, 1% oxygen (hypoxia), and hypoxia with TNF-α-supplemented media and found the highest ECFC attachment on HUVECs stimulated with TNF-α and hypoxia, correlating with the highest ICAM-1 expression. We next designed, fabricated and tested a three-dimensional microbioreactor (3D MBR) system with precise control and monitoring of dissolve oxygen and media flow rate in the cellular environment. We utilized a step-wise seeding approach, producing monolayer of HUVECs on all four walls. When stimulated with both TNF-α and hypoxia, ECFC retention on HUVECs was significantly increased under low shear stress compared to static controls. Overall, the 3D MBR system mimics the pathological oxygen tension and shear stress in the damaged vasculature, providing a platform to model vascular-related disorders.

Duke Scholars

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Published In

Biofabrication

DOI

EISSN

1758-5090

ISSN

1758-5082

Publication Date

December 2015

Volume

7

Issue

4

Start / End Page

045010

Related Subject Headings

  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • Oxygen
  • Models, Biological
  • Microfluidics
  • Intercellular Adhesion Molecule-1
  • Humans
  • Human Umbilical Vein Endothelial Cells
  • Endothelial Progenitor Cells
  • Computer Simulation
 

Citation

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Lewis, D. M., Abaci, H. E., Xu, Y., & Gerecht, S. (2015). Endothelial progenitor cell recruitment in a microfluidic vascular model. Biofabrication, 7(4), 045010. https://doi.org/10.1088/1758-5090/7/4/045010
Lewis, Daniel M., Hasan E. Abaci, Yu Xu, and Sharon Gerecht. “Endothelial progenitor cell recruitment in a microfluidic vascular model.Biofabrication 7, no. 4 (December 2015): 045010. https://doi.org/10.1088/1758-5090/7/4/045010.
Lewis DM, Abaci HE, Xu Y, Gerecht S. Endothelial progenitor cell recruitment in a microfluidic vascular model. Biofabrication. 2015 Dec;7(4):045010.
Lewis, Daniel M., et al. “Endothelial progenitor cell recruitment in a microfluidic vascular model.Biofabrication, vol. 7, no. 4, Dec. 2015, p. 045010. Epmc, doi:10.1088/1758-5090/7/4/045010.
Lewis DM, Abaci HE, Xu Y, Gerecht S. Endothelial progenitor cell recruitment in a microfluidic vascular model. Biofabrication. 2015 Dec;7(4):045010.

Published In

Biofabrication

DOI

EISSN

1758-5090

ISSN

1758-5082

Publication Date

December 2015

Volume

7

Issue

4

Start / End Page

045010

Related Subject Headings

  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • Oxygen
  • Models, Biological
  • Microfluidics
  • Intercellular Adhesion Molecule-1
  • Humans
  • Human Umbilical Vein Endothelial Cells
  • Endothelial Progenitor Cells
  • Computer Simulation