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Engineered human vascularized constructs accelerate diabetic wound healing.

Publication ,  Journal Article
Shen, Y-I; Cho, H; Papa, AE; Burke, JA; Chan, XY; Duh, EJ; Gerecht, S
Published in: Biomaterials
September 2016

Stem cell-based therapy is emerging as a promising approach for chronic diabetic wounds, but strategies for optimizing both cellular differentiation and delivery remain as major obstacles. Here, we study bioengineered vascularized constructs as a therapeutic modality for diabetic wound healing. We developed a wound model in immunodeficient rodent and treated it with engineered vascularized constructs from endothelial progenitors or early vascular cells-derived from human induced pluripotent stem cells (hiPSCs) reprogrammed either from healthy donor or type-1 diabetic patient. We found that all vascularized constructs expedited wound closure and reperfusion, with endothelial progenitor constructs having the earliest maximum closure rate followed closely by healthy and diabetic hiPSC-derivative constructs. This was accompanied by rapid granulation layer formation and regression in all vascularized construct groups. Macrophage infiltration into the hydrogel matrix occurred during early stages of healing, seeming to facilitate rapid neovascularization of the wound that could then better persist in the vascularized constructs. Blood perfusion of the human vasculature could be detected after three days, indicating rapid integration with the host vasculature. Overall, we propose a potential therapeutic strategy using allograft or autologous vascularized constructs to treat type-1 diabetic wounds. This approach highlights the unprecedented prospects of designing patient-specific stem cell therapy.

Duke Scholars

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

September 2016

Volume

102

Start / End Page

107 / 119

Related Subject Headings

  • Wound Healing
  • Tissue Scaffolds
  • Stem Cell Transplantation
  • Neovascularization, Physiologic
  • Mice, Nude
  • Induced Pluripotent Stem Cells
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Hyaluronic Acid
  • Humans
  • Female
 

Citation

APA
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ICMJE
MLA
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Shen, Y.-I., Cho, H., Papa, A. E., Burke, J. A., Chan, X. Y., Duh, E. J., & Gerecht, S. (2016). Engineered human vascularized constructs accelerate diabetic wound healing. Biomaterials, 102, 107–119. https://doi.org/10.1016/j.biomaterials.2016.06.009
Shen, Yu-I, Hongkwan Cho, Arianne E. Papa, Jacqueline A. Burke, Xin Yi Chan, Elia J. Duh, and Sharon Gerecht. “Engineered human vascularized constructs accelerate diabetic wound healing.Biomaterials 102 (September 2016): 107–19. https://doi.org/10.1016/j.biomaterials.2016.06.009.
Shen Y-I, Cho H, Papa AE, Burke JA, Chan XY, Duh EJ, et al. Engineered human vascularized constructs accelerate diabetic wound healing. Biomaterials. 2016 Sep;102:107–19.
Shen, Yu-I., et al. “Engineered human vascularized constructs accelerate diabetic wound healing.Biomaterials, vol. 102, Sept. 2016, pp. 107–19. Epmc, doi:10.1016/j.biomaterials.2016.06.009.
Shen Y-I, Cho H, Papa AE, Burke JA, Chan XY, Duh EJ, Gerecht S. Engineered human vascularized constructs accelerate diabetic wound healing. Biomaterials. 2016 Sep;102:107–119.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

September 2016

Volume

102

Start / End Page

107 / 119

Related Subject Headings

  • Wound Healing
  • Tissue Scaffolds
  • Stem Cell Transplantation
  • Neovascularization, Physiologic
  • Mice, Nude
  • Induced Pluripotent Stem Cells
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Hyaluronic Acid
  • Humans
  • Female