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Micropattern size-dependent endothelial differentiation from a human induced pluripotent stem cell line.

Publication ,  Journal Article
Kusuma, S; Smith, Q; Facklam, A; Gerecht, S
Published in: Journal of tissue engineering and regenerative medicine
March 2017

The multifaceted extracellular milieu presents biochemical and biophysical stimuli that influence stem cell differentiation. Two-dimensional (2D) micropatterned substrates allow the presentation of these cues in spatially defined geometries that have been demonstrated to guide stem cell fate decisions. Leveraging stem cells to reconstruct microvasculature, made up of an inner lining of endothelial cells (ECs) supported by pericytes, is critical to tissue-engineering advances; thus, methods to improve endothelial differentiation efficiency are vital to these efforts. In this study, we examine the hypothesis that the diameter of micropatterned islands influences endothelial differentiation from human induced pluripotent stem cells (hiPSCs). Comparing island diameters of 80, 140, 225 and 500 µm, we found that co-cultures of control ECs and pericytes did not yield variable ratios of cell types; however, when hiPSCs were differentiated toward a bicellular population of ECs and pericytes on these varying micropattern feature sizes, we found that smaller islands promoted EC differentiation efficiency, yielding a derived population composed of 70% ECs, which exhibited a greater sprouting propensity. Differentiation on the largest feature size exhibited a smaller EC yield, similar to that on non-patterned substrates. Taken together, these data demonstrate that micropatterned islands of varying diameters can be used to modulate EC differentiation efficiency. Copyright © 2015 John Wiley & Sons, Ltd.

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Published In

Journal of tissue engineering and regenerative medicine

DOI

EISSN

1932-7005

ISSN

1932-6254

Publication Date

March 2017

Volume

11

Issue

3

Start / End Page

855 / 861

Related Subject Headings

  • Pericytes
  • Neovascularization, Physiologic
  • Induced Pluripotent Stem Cells
  • Humans
  • Human Umbilical Vein Endothelial Cells
  • Coculture Techniques
  • Cell Differentiation
  • Biomedical Engineering
  • 4003 Biomedical engineering
  • 1116 Medical Physiology
 

Citation

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Kusuma, S., Smith, Q., Facklam, A., & Gerecht, S. (2017). Micropattern size-dependent endothelial differentiation from a human induced pluripotent stem cell line. Journal of Tissue Engineering and Regenerative Medicine, 11(3), 855–861. https://doi.org/10.1002/term.1985
Kusuma, Sravanti, Quinton Smith, Amanda Facklam, and Sharon Gerecht. “Micropattern size-dependent endothelial differentiation from a human induced pluripotent stem cell line.Journal of Tissue Engineering and Regenerative Medicine 11, no. 3 (March 2017): 855–61. https://doi.org/10.1002/term.1985.
Kusuma S, Smith Q, Facklam A, Gerecht S. Micropattern size-dependent endothelial differentiation from a human induced pluripotent stem cell line. Journal of tissue engineering and regenerative medicine. 2017 Mar;11(3):855–61.
Kusuma, Sravanti, et al. “Micropattern size-dependent endothelial differentiation from a human induced pluripotent stem cell line.Journal of Tissue Engineering and Regenerative Medicine, vol. 11, no. 3, Mar. 2017, pp. 855–61. Epmc, doi:10.1002/term.1985.
Kusuma S, Smith Q, Facklam A, Gerecht S. Micropattern size-dependent endothelial differentiation from a human induced pluripotent stem cell line. Journal of tissue engineering and regenerative medicine. 2017 Mar;11(3):855–861.

Published In

Journal of tissue engineering and regenerative medicine

DOI

EISSN

1932-7005

ISSN

1932-6254

Publication Date

March 2017

Volume

11

Issue

3

Start / End Page

855 / 861

Related Subject Headings

  • Pericytes
  • Neovascularization, Physiologic
  • Induced Pluripotent Stem Cells
  • Humans
  • Human Umbilical Vein Endothelial Cells
  • Coculture Techniques
  • Cell Differentiation
  • Biomedical Engineering
  • 4003 Biomedical engineering
  • 1116 Medical Physiology