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Spatial control of cell-mediated degradation to regulate vasculogenesis and angiogenesis in hyaluronan hydrogels.

Publication ,  Journal Article
Hanjaya-Putra, D; Wong, KT; Hirotsu, K; Khetan, S; Burdick, JA; Gerecht, S
Published in: Biomaterials
September 2012

Matrix remodeling is crucial for neovascularization, however its utilization to control this process in synthetic biomaterials has been limited. Here, we utilized hyaluronic acid (HA) hydrogels to spatially control cellular remodeling during vascular network formation. Specifically, we exploited a secondary radical polymerization to alter the ability of cells to degrade the hydrogel and utilized it to create spatial patterning using light initiation. We first demonstrated the ability of the hydrogel to either support or inhibit in vitro vasculogenesis of endothelial colony-forming cells (ECFCs) or angiogenesis from ex ovo chorioallantoic membranes. We showed that vascular tube branching and sprouting, which required matrix metalloproteinases (MMPs)-dependent remodeling, could be achieved in hydrogels formed by primary addition-crosslinking only. Although ECFCs expressed higher levels of MMPs in the hydrogels with the secondary radical crosslinking, the generated kinetic chains disabled cell-mediated remodeling and therefore vascular formation was arrested at the vacuole and lumen stage. We then patterned hydrogels to have regions that either permitted or inhibited cell-mediated degradation during in vitro vasculogenesis or angiogenesis. Our ability to control degradation cues that regulate vascular tube formation is important for the study of vascular biology and the application of synthetic biomaterials in tissue regeneration.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

September 2012

Volume

33

Issue

26

Start / End Page

6123 / 6131

Related Subject Headings

  • Tissue Engineering
  • Neovascularization, Physiologic
  • Hydrogels
  • Hyaluronic Acid
  • Humans
  • Cells, Cultured
  • Biomedical Engineering
 

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Hanjaya-Putra, D., Wong, K. T., Hirotsu, K., Khetan, S., Burdick, J. A., & Gerecht, S. (2012). Spatial control of cell-mediated degradation to regulate vasculogenesis and angiogenesis in hyaluronan hydrogels. Biomaterials, 33(26), 6123–6131. https://doi.org/10.1016/j.biomaterials.2012.05.027
Hanjaya-Putra, Donny, Kyle T. Wong, Kelsey Hirotsu, Sudhir Khetan, Jason A. Burdick, and Sharon Gerecht. “Spatial control of cell-mediated degradation to regulate vasculogenesis and angiogenesis in hyaluronan hydrogels.Biomaterials 33, no. 26 (September 2012): 6123–31. https://doi.org/10.1016/j.biomaterials.2012.05.027.
Hanjaya-Putra D, Wong KT, Hirotsu K, Khetan S, Burdick JA, Gerecht S. Spatial control of cell-mediated degradation to regulate vasculogenesis and angiogenesis in hyaluronan hydrogels. Biomaterials. 2012 Sep;33(26):6123–31.
Hanjaya-Putra, Donny, et al. “Spatial control of cell-mediated degradation to regulate vasculogenesis and angiogenesis in hyaluronan hydrogels.Biomaterials, vol. 33, no. 26, Sept. 2012, pp. 6123–31. Epmc, doi:10.1016/j.biomaterials.2012.05.027.
Hanjaya-Putra D, Wong KT, Hirotsu K, Khetan S, Burdick JA, Gerecht S. Spatial control of cell-mediated degradation to regulate vasculogenesis and angiogenesis in hyaluronan hydrogels. Biomaterials. 2012 Sep;33(26):6123–6131.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

September 2012

Volume

33

Issue

26

Start / End Page

6123 / 6131

Related Subject Headings

  • Tissue Engineering
  • Neovascularization, Physiologic
  • Hydrogels
  • Hyaluronic Acid
  • Humans
  • Cells, Cultured
  • Biomedical Engineering