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Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds.

Publication ,  Journal Article
Gerecht, S; Ferreira, LS; Langer, R
Published in: Methods in molecular biology (Clifton, N.J.)
January 2010

The vascularization of tissue constructs remains a major challenge in regenerative medicine, as the diffusional supply of oxygen can support only 100-200 mum thick layers of viable tissue. The formation of a mature and functional vascular network requires communication between endothelial cells (ECs) and smooth muscle cells (SMCs). Potential sources of these cells that involve noninvasive methodologies are required for numerous applications including tissue-engineered vascular grafts, myocardial ischemia, wound healing, plastic surgery, and general tissue-engineering applications. Human embryonic stem cells (hESCs) can be an unlimited source of these cells. They can be expanded in vitro in an undifferentiated state without apparent limit, and hES-derived cells can be created in virtually unlimited amounts for potential clinical uses. Recently, vascular progenitor cells as well as endothelial and smooth muscle cells have been isolated from hESCs.

Duke Scholars

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2010

Volume

584

Start / End Page

333 / 354

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Myocytes, Smooth Muscle
  • Microscopy, Electron, Scanning
  • Mice
  • Hydrogels
  • Hyaluronic Acid
  • Humans
  • Flow Cytometry
  • Endothelial Cells
 

Citation

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Gerecht, S., Ferreira, L. S., & Langer, R. (2010). Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds. Methods in Molecular Biology (Clifton, N.J.), 584, 333–354. https://doi.org/10.1007/978-1-60761-369-5_18
Gerecht, Sharon, Lino S. Ferreira, and Robert Langer. “Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds.Methods in Molecular Biology (Clifton, N.J.) 584 (January 2010): 333–54. https://doi.org/10.1007/978-1-60761-369-5_18.
Gerecht S, Ferreira LS, Langer R. Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds. Methods in molecular biology (Clifton, NJ). 2010 Jan;584:333–54.
Gerecht, Sharon, et al. “Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds.Methods in Molecular Biology (Clifton, N.J.), vol. 584, Jan. 2010, pp. 333–54. Epmc, doi:10.1007/978-1-60761-369-5_18.
Gerecht S, Ferreira LS, Langer R. Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds. Methods in molecular biology (Clifton, NJ). 2010 Jan;584:333–354.

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2010

Volume

584

Start / End Page

333 / 354

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Myocytes, Smooth Muscle
  • Microscopy, Electron, Scanning
  • Mice
  • Hydrogels
  • Hyaluronic Acid
  • Humans
  • Flow Cytometry
  • Endothelial Cells