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Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures.

Publication ,  Journal Article
Yang, Y; Lu, Q; Chen, Y; DeLuca, M; Arya, G; Ke, Y; Zauscher, S
Published in: Angewandte Chemie (International ed. in English)
November 2023

DNA nanotechnology provides an approach to create precise, tunable, and biocompatible nanostructures for biomedical applications. However, the stability of these structures is severely compromised in biological milieu due to their fast degradation by nucleases. Recently, we showed how enzymatic polymerization could be harnessed to grow polynucleotide brushes of tunable length and location on the surface of DNA origami nanostructures, which greatly enhances their nuclease stability. Here, we report on strategies that allow for both spatial and temporal control over polymerization through activatable initiation, cleavage, and regeneration of polynucleotide brushes using restriction enzymes. The ability to site-specifically decorate DNA origami nanostructures with polynucleotide brushes in a spatiotemporally controlled way provides access to "smart" functionalized DNA architectures with potential applications in drug delivery and supramolecular assembly.

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Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

November 2023

Volume

62

Issue

48

Start / End Page

e202311727

Related Subject Headings

  • Polynucleotides
  • Organic Chemistry
  • Nucleic Acid Conformation
  • Nanotechnology
  • Nanostructures
  • Drug Delivery Systems
  • DNA
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

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Yang, Y., Lu, Q., Chen, Y., DeLuca, M., Arya, G., Ke, Y., & Zauscher, S. (2023). Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures. Angewandte Chemie (International Ed. in English), 62(48), e202311727. https://doi.org/10.1002/anie.202311727
Yang, Yunqi, Qinyi Lu, Yu Chen, Marcello DeLuca, Gaurav Arya, Yonggang Ke, and Stefan Zauscher. “Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures.Angewandte Chemie (International Ed. in English) 62, no. 48 (November 2023): e202311727. https://doi.org/10.1002/anie.202311727.
Yang Y, Lu Q, Chen Y, DeLuca M, Arya G, Ke Y, et al. Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures. Angewandte Chemie (International ed in English). 2023 Nov;62(48):e202311727.
Yang, Yunqi, et al. “Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures.Angewandte Chemie (International Ed. in English), vol. 62, no. 48, Nov. 2023, p. e202311727. Epmc, doi:10.1002/anie.202311727.
Yang Y, Lu Q, Chen Y, DeLuca M, Arya G, Ke Y, Zauscher S. Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures. Angewandte Chemie (International ed in English). 2023 Nov;62(48):e202311727.
Journal cover image

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

November 2023

Volume

62

Issue

48

Start / End Page

e202311727

Related Subject Headings

  • Polynucleotides
  • Organic Chemistry
  • Nucleic Acid Conformation
  • Nanotechnology
  • Nanostructures
  • Drug Delivery Systems
  • DNA
  • 34 Chemical sciences
  • 03 Chemical Sciences