Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures.
DNA nanotechnology provides an approach to create precise, tunable, and biocompatible nanostructures for biomedical applications. However, the stability of these structures is severely compromised in biological milieu due to their fast degradation by nucleases. Recently, we showed how enzymatic polymerization could be harnessed to grow polynucleotide brushes of tunable length and location on the surface of DNA origami nanostructures, which greatly enhances their nuclease stability. Here, we report on strategies that allow for both spatial and temporal control over polymerization through activatable initiation, cleavage, and regeneration of polynucleotide brushes using restriction enzymes. The ability to site-specifically decorate DNA origami nanostructures with polynucleotide brushes in a spatiotemporally controlled way provides access to "smart" functionalized DNA architectures with potential applications in drug delivery and supramolecular assembly.
Duke Scholars
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Related Subject Headings
- Polynucleotides
- Organic Chemistry
- Nucleic Acid Conformation
- Nanotechnology
- Nanostructures
- Drug Delivery Systems
- DNA
- 34 Chemical sciences
- 03 Chemical Sciences
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Polynucleotides
- Organic Chemistry
- Nucleic Acid Conformation
- Nanotechnology
- Nanostructures
- Drug Delivery Systems
- DNA
- 34 Chemical sciences
- 03 Chemical Sciences