Skip to main content

The effect of common paralytic agents used for fluorescence imaging on redox tone and ATP levels in Caenorhabditis elegans.

Publication ,  Journal Article
Morton, KS; Wahl, AK; Meyer, JN
Published in: bioRxiv
September 23, 2023

One aspect of Caenorhabditis elegans that makes it a highly valuable model organism is the ease of use of in vivo genetic reporters, facilitated by its transparent cuticle and highly tractable genetics. Despite the rapid advancement of these technologies, worms must be paralyzed for most imaging applications, and few investigations have characterized the impacts of common chemical anesthetic methods on the parameters measured, in particular biochemical measurements such as cellular energetics and redox tone. Using two dynamic reporters, QUEEN-2m for relative ATP levels and reduction-oxidation sensitive GFP (roGFP) for redox tone, we assess the impact of commonly used chemical paralytics. We report that no chemical anesthetic is entirely effective at doses required for full paralysis without altering redox tone or ATP levels, though 100 mM 2,3-Butadione monoxime appears to be the least problematic. We also assess the use of cold shock, commonly used in combination with physical restraint methods, and find that cold shock does not alter either ATP levels or redox tone. In addition to informing which paralytics are most appropriate for research in these topics, we highlight the need for tailoring the use of anesthetics to different endpoints and experimental questions. Further, we reinforce the need for developing less disruptive paralytic methods for optimal imaging of dynamic in vivo reporters.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

bioRxiv

DOI

Publication Date

September 23, 2023

Location

United States
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Morton, K. S., Wahl, A. K., & Meyer, J. N. (2023). The effect of common paralytic agents used for fluorescence imaging on redox tone and ATP levels in Caenorhabditis elegans. BioRxiv. https://doi.org/10.1101/2023.09.21.558750
Morton, Katherine S., Ashlyn K. Wahl, and Joel N. Meyer. “The effect of common paralytic agents used for fluorescence imaging on redox tone and ATP levels in Caenorhabditis elegans.BioRxiv, September 23, 2023. https://doi.org/10.1101/2023.09.21.558750.
Morton, Katherine S., et al. “The effect of common paralytic agents used for fluorescence imaging on redox tone and ATP levels in Caenorhabditis elegans.BioRxiv, Sept. 2023. Pubmed, doi:10.1101/2023.09.21.558750.

Published In

bioRxiv

DOI

Publication Date

September 23, 2023

Location

United States