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Human Immune Cell Epigenomic Signatures in Response to Infectious Diseases and Chemical Exposures.

Publication ,  Journal Article
Wang, W; Hariharan, M; Bartlett, A; Barragan, C; Castanon, R; Rothenberg, V; Song, H; Nery, J; Aldridge, A; Altshul, J; Kenworthy, M; Ding, W ...
Published in: bioRxiv
June 30, 2023

Variations in DNA methylation patterns in human tissues have been linked to various environmental exposures and infections. Here, we identified the DNA methylation signatures associated with multiple exposures in nine major immune cell types derived from peripheral blood mononuclear cells (PBMCs) at single-cell resolution. We performed methylome sequencing on 111,180 immune cells obtained from 112 individuals who were exposed to different viruses, bacteria, or chemicals. Our analysis revealed 790,662 differentially methylated regions (DMRs) associated with these exposures, which are mostly individual CpG sites. Additionally, we integrated methylation and ATAC-seq data from same samples and found strong correlations between the two modalities. However, the epigenomic remodeling in these two modalities are complementary. Finally, we identified the minimum set of DMRs that can predict exposures. Overall, our study provides the first comprehensive dataset of single immune cell methylation profiles, along with unique methylation biomarkers for various biological and chemical exposures.

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bioRxiv

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Publication Date

June 30, 2023

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United States
 

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Wang, W., Hariharan, M., Bartlett, A., Barragan, C., Castanon, R., Rothenberg, V., … Ecker, J. R. (2023). Human Immune Cell Epigenomic Signatures in Response to Infectious Diseases and Chemical Exposures. BioRxiv. https://doi.org/10.1101/2023.06.29.546792
Wang, Wenliang, Manoj Hariharan, Anna Bartlett, Cesar Barragan, Rosa Castanon, Vince Rothenberg, Haili Song, et al. “Human Immune Cell Epigenomic Signatures in Response to Infectious Diseases and Chemical Exposures.BioRxiv, June 30, 2023. https://doi.org/10.1101/2023.06.29.546792.
Wang W, Hariharan M, Bartlett A, Barragan C, Castanon R, Rothenberg V, et al. Human Immune Cell Epigenomic Signatures in Response to Infectious Diseases and Chemical Exposures. bioRxiv. 2023 Jun 30;
Wang, Wenliang, et al. “Human Immune Cell Epigenomic Signatures in Response to Infectious Diseases and Chemical Exposures.BioRxiv, June 2023. Pubmed, doi:10.1101/2023.06.29.546792.
Wang W, Hariharan M, Bartlett A, Barragan C, Castanon R, Rothenberg V, Song H, Nery J, Aldridge A, Altshul J, Kenworthy M, Ding W, Liu H, Tian W, Zhou J, Chen H, Wei B, Gündüz IB, Norell T, Broderick TJ, McClain MT, Satterwhite LL, Burke TW, Petzold EA, Shen X, Woods CW, Fowler VG, Ruffin F, Panuwet P, Barr DB, Beare JL, Smith AK, Spurbeck RR, Vangeti S, Ramos I, Nudelman G, Sealfon SC, Castellino F, Walley AM, Evans T, Müller F, Greenleaf WJ, Ecker JR. Human Immune Cell Epigenomic Signatures in Response to Infectious Diseases and Chemical Exposures. bioRxiv. 2023 Jun 30;

Published In

bioRxiv

DOI

Publication Date

June 30, 2023

Location

United States