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Citrus peel extract protects against diesel exhaust particle-induced chronic obstructive pulmonary disease-like lung lesions and oxidative stress.

Publication ,  Journal Article
Fang, Z-F; Fu, Y; Peng, Y; Song, S-R; Wang, Z-N; Yang, Y; Nie, Y-C; Han, H-L; Teng, Y-B; Xiao, W-M; Chen, J-P; Zhou, B-J; Ou, G-L; Xie, J-X ...
Published in: Food & function
October 2023

Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide and characterized by emphysema, small airway remodeling and mucus hypersecretion. Citrus peels have been widely used as food spices and in traditional Chinese medicine for chronic lung disease. Given that citrus peels are known for containing antioxidants and anti-inflammatory compounds, we hypothesize that citrus peel intake can suppress oxidative stress and inflammatory response to air pollution exposure, thereby alleviating COPD-like pathologies. This study aimed to investigate the efficacy of citrus peel extract, namely Guang Chenpi (GC), in preventing the development of COPD induced by diesel exhaust particles (DEPs) and its potential mechanism. DEP-induced COPD-like lung pathologies, inflammatory responses and oxidative stress with or without GC treatment were examined in vivo and in vitro. Our in vivo study showed that GC was effective in decreasing inflammatory cell counts and inflammatory mediator (IL-17A and TNF-α) concentrations in bronchoalveolar lavage fluid (BALF). Pretreatment with GC extract also significantly decreased oxidative stress in the serum and lung tissue of DEP-induced COPD rats. Furthermore, GC pretreatment effectively reduced goblet cell hyperplasia (PAS positive cells) and fibrosis of the small airways, decreased macrophage infiltration as well as carbon loading in the peripheral lungs, and facilitated the resolution of emphysema and small airway remodeling in DEP-induced COPD rats. An in vitro free radical scavenging assay revealed robust antioxidant potential of GC in scavenging DPPH free radicals. Moreover, GC demonstrated potent capacities in reducing ROS production and enhancing SOD activity in BEAS-2B cells stimulated by DEPs. GC treatment significantly attenuated the increased level of IL-8 and MUC5AC from DEP-treated BEAS-2B cells. Mechanistically, GC treatment upregulated the protein level of Nrf-2 and could function via MAPK/NF-κB signaling pathways by suppressing the phosphorylation of p38, JNK and p65. Citrus peel extract is effective in decreasing oxidative stress and inflammatory responses of the peripheral lungs to DEP exposure. These protective effects further contributed to the resolution of COPD-like pathologies.

Duke Scholars

Published In

Food & function

DOI

EISSN

2042-650X

ISSN

2042-6496

Publication Date

October 2023

Volume

14

Issue

21

Start / End Page

9841 / 9856

Related Subject Headings

  • Vehicle Emissions
  • Rats
  • Pulmonary Disease, Chronic Obstructive
  • Oxidative Stress
  • Lung
  • Emphysema
  • Citrus
  • Bronchoalveolar Lavage Fluid
  • Antioxidants
  • Animals
 

Citation

APA
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Fang, Z.-F., Fu, Y., Peng, Y., Song, S.-R., Wang, Z.-N., Yang, Y., … Zhong, N.-S. (2023). Citrus peel extract protects against diesel exhaust particle-induced chronic obstructive pulmonary disease-like lung lesions and oxidative stress. Food & Function, 14(21), 9841–9856. https://doi.org/10.1039/d3fo02010j
Fang, Zhang-Fu, Yu Fu, Yang Peng, Sheng-Ren Song, Zhao-Ni Wang, Yang Yang, Yi-Chu Nie, et al. “Citrus peel extract protects against diesel exhaust particle-induced chronic obstructive pulmonary disease-like lung lesions and oxidative stress.Food & Function 14, no. 21 (October 2023): 9841–56. https://doi.org/10.1039/d3fo02010j.
Fang Z-F, Fu Y, Peng Y, Song S-R, Wang Z-N, Yang Y, et al. Citrus peel extract protects against diesel exhaust particle-induced chronic obstructive pulmonary disease-like lung lesions and oxidative stress. Food & function. 2023 Oct;14(21):9841–56.
Fang, Zhang-Fu, et al. “Citrus peel extract protects against diesel exhaust particle-induced chronic obstructive pulmonary disease-like lung lesions and oxidative stress.Food & Function, vol. 14, no. 21, Oct. 2023, pp. 9841–56. Epmc, doi:10.1039/d3fo02010j.
Fang Z-F, Fu Y, Peng Y, Song S-R, Wang Z-N, Yang Y, Nie Y-C, Han H-L, Teng Y-B, Xiao W-M, Chen J-P, Zhou B-J, Ou G-L, Xie J-X, Liu X-Y, Zhang JJ, Zhong N-S. Citrus peel extract protects against diesel exhaust particle-induced chronic obstructive pulmonary disease-like lung lesions and oxidative stress. Food & function. 2023 Oct;14(21):9841–9856.
Journal cover image

Published In

Food & function

DOI

EISSN

2042-650X

ISSN

2042-6496

Publication Date

October 2023

Volume

14

Issue

21

Start / End Page

9841 / 9856

Related Subject Headings

  • Vehicle Emissions
  • Rats
  • Pulmonary Disease, Chronic Obstructive
  • Oxidative Stress
  • Lung
  • Emphysema
  • Citrus
  • Bronchoalveolar Lavage Fluid
  • Antioxidants
  • Animals