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A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections

Publication ,  Journal Article
Schoenmakers, JWA; López‑Álvarez, M; IJpma, FFA; Wouthuyzen-Bakker, M; McNamara, JO; van Oosten, M; Jutte, PC; van Dijl, JM
Published in: European Journal of Nuclear Medicine and Molecular Imaging
January 1, 2023

Purpose: Staphylococcus aureus is the most common and impactful multi-drug resistant pathogen implicated in (periprosthetic) joint infections (PJI) and fracture-related infections (FRI). Therefore, the present proof-of-principle study was aimed at the rapid detection of S. aureus in synovial fluids and biofilms on extracted osteosynthesis materials through bacteria-targeted fluorescence imaging with the ‘smart-activatable’ DNA-based AttoPolyT probe. This fluorogenic oligonucleotide probe yields large fluorescence increases upon cleavage by micrococcal nuclease, an enzyme secreted by S. aureus. Methods: Synovial fluids from patients with suspected PJI and extracted osteosynthesis materials from trauma patients with suspected FRI were inspected for S. aureus nuclease activity with the AttoPolyT probe. Biofilms on osteosynthesis materials were imaged with the AttoPolyT probe and a vancomycin-IRDye800CW conjugate (vanco-800CW) specific for Gram-positive bacteria. Results: 38 synovial fluid samples were collected and analyzed. Significantly higher fluorescence levels were measured for S. aureus-positive samples compared to, respectively, other Gram-positive bacterial pathogens (p < 0.0001), Gram-negative bacterial pathogens (p = 0.0038) and non-infected samples (p = 0.0030), allowing a diagnosis of S. aureus-associated PJI within 2 h. Importantly, S. aureus-associated biofilms on extracted osteosynthesis materials from patients with FRI were accurately imaged with the AttoPolyT probe, allowing their correct distinction from biofilms formed by other Gram-positive bacteria detected with vanco-800CW within 15 min. Conclusion: The present study highlights the potential clinical value of the AttoPolyT probe for fast and accurate detection of S. aureus infection in synovial fluids and biofilms on extracted osteosynthesis materials.

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Published In

European Journal of Nuclear Medicine and Molecular Imaging

DOI

EISSN

1619-7089

ISSN

1619-7070

Publication Date

January 1, 2023

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
  • 0299 Other Physical Sciences
 

Citation

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Schoenmakers, J. W. A., López‑Álvarez, M., IJpma, F. F. A., Wouthuyzen-Bakker, M., McNamara, J. O., van Oosten, M., … van Dijl, J. M. (2023). A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections. European Journal of Nuclear Medicine and Molecular Imaging. https://doi.org/10.1007/s00259-023-06499-4
Schoenmakers, J. W. A., M. López‑Álvarez, F. F. A. IJpma, M. Wouthuyzen-Bakker, J. O. McNamara, M. van Oosten, P. C. Jutte, and J. M. van Dijl. “A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections.” European Journal of Nuclear Medicine and Molecular Imaging, January 1, 2023. https://doi.org/10.1007/s00259-023-06499-4.
Schoenmakers JWA, López‑Álvarez M, IJpma FFA, Wouthuyzen-Bakker M, McNamara JO, van Oosten M, et al. A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections. European Journal of Nuclear Medicine and Molecular Imaging. 2023 Jan 1;
Schoenmakers, J. W. A., et al. “A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections.” European Journal of Nuclear Medicine and Molecular Imaging, Jan. 2023. Scopus, doi:10.1007/s00259-023-06499-4.
Schoenmakers JWA, López‑Álvarez M, IJpma FFA, Wouthuyzen-Bakker M, McNamara JO, van Oosten M, Jutte PC, van Dijl JM. A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections. European Journal of Nuclear Medicine and Molecular Imaging. 2023 Jan 1;
Journal cover image

Published In

European Journal of Nuclear Medicine and Molecular Imaging

DOI

EISSN

1619-7089

ISSN

1619-7070

Publication Date

January 1, 2023

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
  • 0299 Other Physical Sciences