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Dynamical control enables the formation of demixed biomolecular condensates.

Publication ,  Journal Article
Lin, AZ; Ruff, KM; Dar, F; Jalihal, A; King, MR; Lalmansingh, JM; Posey, AE; Erkamp, NA; Seim, I; Gladfelter, AS; Pappu, RV
Published in: Nat Commun
November 24, 2023

Cellular matter can be organized into compositionally distinct biomolecular condensates. For example, in Ashbya gossypii, the RNA-binding protein Whi3 forms distinct condensates with different RNA molecules. Using criteria derived from a physical framework for explaining how compositionally distinct condensates can form spontaneously via thermodynamic considerations, we find that condensates in vitro form mainly via heterotypic interactions in binary mixtures of Whi3 and RNA. However, within these condensates, RNA molecules become dynamically arrested. As a result, in ternary systems, simultaneous additions of Whi3 and pairs of distinct RNA molecules lead to well-mixed condensates, whereas delayed addition of an RNA component results in compositional distinctness. Therefore, compositional identities of condensates can be achieved via dynamical control, being driven, at least partially, by the dynamical arrest of RNA molecules. Finally, we show that synchronizing the production of different RNAs leads to more well-mixed, as opposed to compositionally distinct condensates in vivo.

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

November 24, 2023

Volume

14

Issue

1

Start / End Page

7678

Location

England

Related Subject Headings

  • Thermodynamics
  • RNA
  • Biomolecular Condensates
 

Citation

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Lin, A. Z., Ruff, K. M., Dar, F., Jalihal, A., King, M. R., Lalmansingh, J. M., … Pappu, R. V. (2023). Dynamical control enables the formation of demixed biomolecular condensates. Nat Commun, 14(1), 7678. https://doi.org/10.1038/s41467-023-43489-4
Lin, Andrew Z., Kiersten M. Ruff, Furqan Dar, Ameya Jalihal, Matthew R. King, Jared M. Lalmansingh, Ammon E. Posey, et al. “Dynamical control enables the formation of demixed biomolecular condensates.Nat Commun 14, no. 1 (November 24, 2023): 7678. https://doi.org/10.1038/s41467-023-43489-4.
Lin AZ, Ruff KM, Dar F, Jalihal A, King MR, Lalmansingh JM, et al. Dynamical control enables the formation of demixed biomolecular condensates. Nat Commun. 2023 Nov 24;14(1):7678.
Lin, Andrew Z., et al. “Dynamical control enables the formation of demixed biomolecular condensates.Nat Commun, vol. 14, no. 1, Nov. 2023, p. 7678. Pubmed, doi:10.1038/s41467-023-43489-4.
Lin AZ, Ruff KM, Dar F, Jalihal A, King MR, Lalmansingh JM, Posey AE, Erkamp NA, Seim I, Gladfelter AS, Pappu RV. Dynamical control enables the formation of demixed biomolecular condensates. Nat Commun. 2023 Nov 24;14(1):7678.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

November 24, 2023

Volume

14

Issue

1

Start / End Page

7678

Location

England

Related Subject Headings

  • Thermodynamics
  • RNA
  • Biomolecular Condensates