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Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling.

Publication ,  Journal Article
Zhang, Y; Yang, H
Published in: Methods Mol Biol
2024

Trophoblast fusion or syncytialization is a fundamental yet poorly understood process during placental development. Primary cultured cytotrophoblasts and human choriocarcinoma cell lines are commonly used to study trophoblast fusion mechanisms in vitro. Quantification of trophoblast fusion index is a key for the in vitro studies. In this chapter, we describe a new method to quantify fusion index, which is based on fluorescent labeling of the plasma membrane using Di-8-ANEPPS, a membrane potential dye. This method directly works on live cells, thereby is simple, economic, and reliable.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2024

Volume

2728

Start / End Page

99 / 104

Location

United States

Related Subject Headings

  • Trophoblasts
  • Pregnancy
  • Placenta
  • Membranes
  • Humans
  • Female
  • Developmental Biology
  • Coloring Agents
  • Cell Membrane
  • Cell Line
 

Citation

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Zhang, Y., & Yang, H. (2024). Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling. Methods Mol Biol, 2728, 99–104. https://doi.org/10.1007/978-1-0716-3495-0_8
Zhang, Yang, and Huanghe Yang. “Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling.Methods Mol Biol 2728 (2024): 99–104. https://doi.org/10.1007/978-1-0716-3495-0_8.
Zhang Y, Yang H. Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling. Methods Mol Biol. 2024;2728:99–104.
Zhang, Yang, and Huanghe Yang. “Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling.Methods Mol Biol, vol. 2728, 2024, pp. 99–104. Pubmed, doi:10.1007/978-1-0716-3495-0_8.
Zhang Y, Yang H. Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling. Methods Mol Biol. 2024;2728:99–104.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2024

Volume

2728

Start / End Page

99 / 104

Location

United States

Related Subject Headings

  • Trophoblasts
  • Pregnancy
  • Placenta
  • Membranes
  • Humans
  • Female
  • Developmental Biology
  • Coloring Agents
  • Cell Membrane
  • Cell Line