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Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans.

Publication ,  Journal Article
Campbell, JX; Schulte, NB; Lai, B; Harris, HH; Franz, KJ
Published in: Metallomics : integrated biometal science
December 2023

Histatin-5 (Hist-5) is an antimicrobial peptide found in human saliva that functions to defend the oral cavity from microbial infections, such as those caused by the fungal pathogen Candida albicans (C. albicans). Hist-5 can bind Cu in multiple oxidation states, Cu2+ and Cu+in vitro, and supplemental Cu2+ has been shown to improve the fungicidal activity of the peptide against C. albicans in culture. However, the exact role of Cu on the antifungal activity of Hist-5 and whether direct peptide-Cu interactions occur intracellularly has yet to be fully determined. Here, we used a combination of fluorescence spectroscopy and confocal microscopy experiments to show reversible Cu-dependent quenching of a fluorescent Hist-5 analogue, Hist-5*, indicating a direct interaction between Hist-5 and intracellular Cu. X-ray fluorescence microscopy images revealed peptide-induced changes to cellular Cu distribution and cell-associated Cu content. These data support a model in which Hist-5 can facilitate the hyperaccumulation of Cu in C. albicans and directly interact with Cu intracellularly to increase the fungicidal activity of Hist-5.

Duke Scholars

Published In

Metallomics : integrated biometal science

DOI

EISSN

1756-591X

ISSN

1756-5901

Publication Date

December 2023

Volume

15

Issue

12

Start / End Page

mfad070

Related Subject Headings

  • Microscopy, Confocal
  • Microbial Sensitivity Tests
  • Humans
  • Histatins
  • Copper
  • Candida albicans
  • Antifungal Agents
  • Analytical Chemistry
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

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ICMJE
MLA
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Campbell, J. X., Schulte, N. B., Lai, B., Harris, H. H., & Franz, K. J. (2023). Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans. Metallomics : Integrated Biometal Science, 15(12), mfad070. https://doi.org/10.1093/mtomcs/mfad070
Campbell, Joanna X., Natalie B. Schulte, Barry Lai, Hugh H. Harris, and Katherine J. Franz. “Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans.Metallomics : Integrated Biometal Science 15, no. 12 (December 2023): mfad070. https://doi.org/10.1093/mtomcs/mfad070.
Campbell JX, Schulte NB, Lai B, Harris HH, Franz KJ. Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans. Metallomics : integrated biometal science. 2023 Dec;15(12):mfad070.
Campbell, Joanna X., et al. “Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans.Metallomics : Integrated Biometal Science, vol. 15, no. 12, Dec. 2023, p. mfad070. Epmc, doi:10.1093/mtomcs/mfad070.
Campbell JX, Schulte NB, Lai B, Harris HH, Franz KJ. Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans. Metallomics : integrated biometal science. 2023 Dec;15(12):mfad070.
Journal cover image

Published In

Metallomics : integrated biometal science

DOI

EISSN

1756-591X

ISSN

1756-5901

Publication Date

December 2023

Volume

15

Issue

12

Start / End Page

mfad070

Related Subject Headings

  • Microscopy, Confocal
  • Microbial Sensitivity Tests
  • Humans
  • Histatins
  • Copper
  • Candida albicans
  • Antifungal Agents
  • Analytical Chemistry
  • 34 Chemical sciences
  • 03 Chemical Sciences