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Encoding Structure in Intrinsically Disordered Protein Biomaterials.

Publication ,  Journal Article
Strader, RL; Shmidov, Y; Chilkoti, A
Published in: Accounts of chemical research
February 2024

ConspectusIn nature, proteins range from those with highly ordered secondary and tertiary structures to those that completely lack a well-defined three-dimensional structure, termed intrinsically disordered proteins (IDPs). IDPs are generally characterized by one or more segments that have a compositional bias toward small hydrophilic amino acids and proline residues that promote structural disorder and are called intrinsically disordered regions (IDRs). The combination of IDRs with ordered regions and the interactions between the two determine the phase behavior, structure, and function of IDPs. Nature also diversifies the structure of proteins and thereby their functions by hybridization of the proteins with other moieties such as glycans and lipids; for instance, post-translationally glycosylated and lipidated proteins are important cell membrane components. Additionally, diversity in protein structure and function is achieved in nature through cross-linking proteins within themselves or with other domains to create various topologies. For example, an essential characteristic of the extracellular matrix (ECM) is the cross-linking of its network components, including proteins such as collagen and elastin, as well as polysaccharides such as hyaluronic acid (HA). Inspired by nature, synthetic IDP (SynIDP)-based biomaterials can be designed by employing similar strategies with the goal of introducing structural diversity and hence unique physiochemical properties. This Account describes such materials produced over the past decade and following one or more of the following approaches: (1) incorporating highly ordered domains into SynIDPs, (2) conjugating SynIDPs to other moieties through either genetically encoded post-translational modification or chemical conjugation, and (3) engineering the topology of SynIDPs via chemical modification. These approaches introduce modifications to the primary structure of SynIDPs, which are then translated to unique three-dimensional secondary and tertiary structures. Beginning with completely disordered SynIDPs as the point of origin, structure may be introduced into SynIDPs by each of these three unique approaches individually along orthogonal axes or by combinations of the three, enabling bioinspired designs to theoretically span the entire range of three-dimensional structural possibilities. Furthermore, the resultant structures span a wide range of length scales, from nano- to meso- to micro- and even macrostructures. In this Account, emphasis is placed on the physiochemical properties and structural features of the described materials. Conjugates of SynIDPs to synthetic polymers and materials achieved by simple mixing of components are outside the scope of this Account. Related biomedical applications are described briefly. Finally, we note future directions for the design of functional SynIDP-based biomaterials.

Duke Scholars

Published In

Accounts of chemical research

DOI

EISSN

1520-4898

ISSN

0001-4842

Publication Date

February 2024

Volume

57

Issue

3

Start / End Page

302 / 311

Related Subject Headings

  • Protein Conformation
  • Intrinsically Disordered Proteins
  • Hyaluronic Acid
  • General Chemistry
  • Amino Acids
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

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ICMJE
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Strader, R. L., Shmidov, Y., & Chilkoti, A. (2024). Encoding Structure in Intrinsically Disordered Protein Biomaterials. Accounts of Chemical Research, 57(3), 302–311. https://doi.org/10.1021/acs.accounts.3c00624
Strader, Rachel L., Yulia Shmidov, and Ashutosh Chilkoti. “Encoding Structure in Intrinsically Disordered Protein Biomaterials.Accounts of Chemical Research 57, no. 3 (February 2024): 302–11. https://doi.org/10.1021/acs.accounts.3c00624.
Strader RL, Shmidov Y, Chilkoti A. Encoding Structure in Intrinsically Disordered Protein Biomaterials. Accounts of chemical research. 2024 Feb;57(3):302–11.
Strader, Rachel L., et al. “Encoding Structure in Intrinsically Disordered Protein Biomaterials.Accounts of Chemical Research, vol. 57, no. 3, Feb. 2024, pp. 302–11. Epmc, doi:10.1021/acs.accounts.3c00624.
Strader RL, Shmidov Y, Chilkoti A. Encoding Structure in Intrinsically Disordered Protein Biomaterials. Accounts of chemical research. 2024 Feb;57(3):302–311.
Journal cover image

Published In

Accounts of chemical research

DOI

EISSN

1520-4898

ISSN

0001-4842

Publication Date

February 2024

Volume

57

Issue

3

Start / End Page

302 / 311

Related Subject Headings

  • Protein Conformation
  • Intrinsically Disordered Proteins
  • Hyaluronic Acid
  • General Chemistry
  • Amino Acids
  • 34 Chemical sciences
  • 03 Chemical Sciences