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A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids.

Publication ,  Journal Article
Shi, C; Yang, D; Ma, X; Pan, L; Shao, Y; Arya, G; Ke, Y; Zhang, C; Wang, F; Zuo, X; Li, M; Wang, P
Published in: Angewandte Chemie (International ed. in English)
March 2024

Nucleic acids in biofluids are emerging biomarkers for the molecular diagnostics of diseases, but their clinical use has been hindered by the lack of sensitive detection assays. Herein, we report the development of a sensitive nucleic acid detection assay named SPOT (sensitive loop-initiated DNAzyme biosensor for nucleic acid detection) by rationally designing a catalytic DNAzyme of endonuclease capability into a unified one-stranded allosteric biosensor. SPOT is activated once a nucleic acid target of a specific sequence binds to its allosteric module to enable continuous cleavage of molecular reporters. SPOT provides a highly robust platform for sensitive, convenient and cost-effective detection of low-abundance nucleic acids. For clinical validation, we demonstrated that SPOT could detect serum miRNAs for the diagnostics of breast cancer, gastric cancer and prostate cancer. Furthermore, SPOT exhibits potent detection performance over SARS-CoV-2 RNA from clinical swabs with high sensitivity and specificity. Finally, SPOT is compatible with point-of-care testing modalities such as lateral flow assays. Hence, we envision that SPOT may serve as a robust assay for the sensitive detection of a variety of nucleic acid targets enabling molecular diagnostics in clinics.

Duke Scholars

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Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

March 2024

Volume

63

Issue

12

Start / End Page

e202320179

Related Subject Headings

  • RNA, Viral
  • Organic Chemistry
  • Nucleic Acid Amplification Techniques
  • MicroRNAs
  • Endonucleases
  • DNA, Catalytic
  • Biosensing Techniques
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

APA
Chicago
ICMJE
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Shi, C., Yang, D., Ma, X., Pan, L., Shao, Y., Arya, G., … Wang, P. (2024). A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids. Angewandte Chemie (International Ed. in English), 63(12), e202320179. https://doi.org/10.1002/anie.202320179
Shi, Chenzhi, Donglei Yang, Xiaowei Ma, Li Pan, Yuanchuan Shao, Gaurav Arya, Yonggang Ke, et al. “A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids.Angewandte Chemie (International Ed. in English) 63, no. 12 (March 2024): e202320179. https://doi.org/10.1002/anie.202320179.
Shi C, Yang D, Ma X, Pan L, Shao Y, Arya G, et al. A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids. Angewandte Chemie (International ed in English). 2024 Mar;63(12):e202320179.
Shi, Chenzhi, et al. “A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids.Angewandte Chemie (International Ed. in English), vol. 63, no. 12, Mar. 2024, p. e202320179. Epmc, doi:10.1002/anie.202320179.
Shi C, Yang D, Ma X, Pan L, Shao Y, Arya G, Ke Y, Zhang C, Wang F, Zuo X, Li M, Wang P. A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids. Angewandte Chemie (International ed in English). 2024 Mar;63(12):e202320179.
Journal cover image

Published In

Angewandte Chemie (International ed. in English)

DOI

EISSN

1521-3773

ISSN

1433-7851

Publication Date

March 2024

Volume

63

Issue

12

Start / End Page

e202320179

Related Subject Headings

  • RNA, Viral
  • Organic Chemistry
  • Nucleic Acid Amplification Techniques
  • MicroRNAs
  • Endonucleases
  • DNA, Catalytic
  • Biosensing Techniques
  • 34 Chemical sciences
  • 03 Chemical Sciences